This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tsai, K J Articles by Lynn, A R Search for Related Content PubMed PubMed Citation Articles by Tsai, K J Articles by Lynn, A R

research-article

ATP-dependent cadmium transport by the cadA cadmium resistance determinant in everted membrane vesicles of Bacillus subtilis.

Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201.

ABSTRACT

Resistance to cadmium conferred by the staphylococcal plasmid pI258 occurs by means of energy-dependent efflux, resulting in decreased intracellular accumulation of cadmium. Recent sequence information suggested that efflux is mediated by a P-type ATPase. The cadA gene was previously expressed in Bacillus subtilis, conferring resistance to cadmium. Everted membrane vesicles were prepared from B. subtilis cells harboring either a plasmid containing the cadA system or the vector plasmid alone. 109Cd2+ transport into the everted membranes was measured in the presence of various energy sources. Cadmium transport was detected only in the presence of ATP as an energy source. The production of an electrochemical proton gradient (delta mu H+) by using NADH or phenazine methosulfate plus ascorbate was not able to drive transport. Reagents which dissipate delta pH abolished calcium transport due to the Ca2+/H+ antiporter but only partially inhibited cadmium transport. Inhibition of transport by the antibiotic bafilomycin A1 occurred at concentrations comparable to those which inhibit P-type ATPases. A band corresponding to the cadA gene product was identified on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and antibodies to the protein were prepared.

This article has been cited by other articles:

• Naz, N., Young, H. K., Ahmed, N., Gadd, G. M. (2005). Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria. Appl. Environ. Microbiol. 71: 4610-4618 [Abstract] [Full Text]  
• Eren, E., Arguello, J. M. (2004). Arabidopsis HMA2, a Divalent Heavy Metal-Transporting PIB-Type ATPase, Is Involved in Cytoplasmic Zn2+ Homeostasis. Plant Physiol. 136: 3712-3723 [Abstract] [Full Text]  
• Mana-Capelli, S., Mandal, A. K., Arguello, J. M. (2003). Archaeoglobus fulgidus CopB Is a Thermophilic Cu2+-ATPase: FUNCTIONAL ROLE OF ITS HISTIDINE-RICH N-TERMINAL METAL BINDING DOMAIN. J. Biol. Chem. 278: 40534-40541 [Abstract] [Full Text]  
• ARGUELLO, J. M., MANDAL, A. K., MANA-CAPELLI, S. (2003). Heavy Metal Transport CPx-ATPases from the Thermophile Archaeoglobus fulgidus. Ann. N. Y. Acad. Sci. 986: 212-218 [Abstract] [Full Text]  
• Wong, M. D., Lin, Y.-F., Rosen, B. P. (2002). The Soft Metal Ion Binding Sites in the Staphylococcus aureus pI258 CadC Cd(II)/Pb(II)/Zn(II)-responsive Repressor Are Formed between Subunits of the Homodimer. J. Biol. Chem. 277: 40930-40936 [Abstract] [Full Text]  
• Tong, L., Nakashima, S., Shibasaka, M., Katsuhara, M., Kasamo, K. (2002). A Novel Histidine-Rich CPx-ATPase from the Filamentous Cyanobacterium Oscillatoria brevis Related to Multiple-Heavy-Metal Cotolerance. J. Bacteriol. 184: 5027-5035 [Abstract] [Full Text]  
• Mandal, A. K., Cheung, W. D., Arguello, J. M. (2002). Characterization of a Thermophilic P-type Ag+/Cu+-ATPase from the Extremophile Archaeoglobus fulgidus. J. Biol. Chem. 277: 7201-7208 [Abstract] [Full Text]  
• Sharma, R., Rensing, C., Rosen, B. P., Mitra, B. (2000). The ATP Hydrolytic Activity of Purified ZntA, a Pb(II)/Cd(II)/Zn(II)-translocating ATPase from Escherichia coli. J. Biol. Chem. 275: 3873-3878 [Abstract] [Full Text]  
• Hao, Z., Chen, S., Wilson, D. B. (1999). Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum. Appl. Environ. Microbiol. 65: 4746-4752 [Abstract] [Full Text]  
• Rensing, C., Ghosh, M., Rosen, B. P. (1999). Families of Soft-Metal-Ion-Transporting ATPases. J. Bacteriol. 181: 5891-5897 [Full Text]  
• Rensing, C., Sun, Y., Mitra, B., Rosen, B. P. (1998). Pb(II)-translocating P-type ATPases. J. Biol. Chem. 273: 32614-32617 [Abstract] [Full Text]  
• Rensing, C., Mitra, B., Rosen, B. P. (1997). The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase. Proc. Natl. Acad. Sci. USA 94: 14326-14331 [Abstract] [Full Text]  
• Solioz, M., Odermatt, A. (1995). Copper and Silver Transport by CopB-ATPase in Membrane Vesicles of Enterococcus hirae. J. Biol. Chem. 270: 9217-9221 [Abstract] [Full Text]  
• Gatti, D., Mitra, B., Rosen, B. P. (2000). Escherichia coli Soft Metal Ion-translocating ATPases. J. Biol. Chem. 275: 34009-34012 [Full Text]