This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ochs, D Articles by Poralla, K Search for Related Content PubMed PubMed Citation Articles by Ochs, D Articles by Poralla, K

research-article

Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolism.

Botanisches Institut, Mikrobiologie, Universität Tübingen, Germany.

ABSTRACT

The pentacyclic hopanoids, a class of eubacterial lipids, are synthesized by squalene-hopene cyclase and side chain-elongating enzymes. With the aid of DNA probes based on the amino-terminal sequence of purified squalene-hopene cyclase from Bacillus acidocaldarius, clones of Escherichia coli that express this enzyme in the cytoplasmic membrane were isolated. According to the DNA sequence, the cyclase contained 627 amino acids with a molecular mass of 69,473 Da. A high percentage of the amino acids were basic. No significant similarity to existing sequenced proteins was found.

This article has been cited by other articles:

• Hamano, Y., Kuzuyama, T., Itoh, N., Furihata, K., Seto, H., Dairi, T. (2002). Functional Analysis of Eubacterial Diterpene Cyclases Responsible for Biosynthesis of a Diterpene Antibiotic, Terpentecin. J. Biol. Chem. 277: 37098-37104 [Abstract] [Full Text]  
• Dairi, T., Hamano, Y., Kuzuyama, T., Itoh, N., Furihata, K., Seto, H. (2001). Eubacterial Diterpene Cyclase Genes Essential for Production of the Isoprenoid Antibiotic Terpentecin. J. Bacteriol. 183: 6085-6094 [Abstract] [Full Text]  
• Kawaide, H., Sassa, T., Kamiya, Y. (2000). Functional Analysis of the Two Interacting Cyclase Domains in ent-Kaurene Synthase from the Fungus Phaeosphaeria sp. L487 and a Comparison with Cyclases from Higher Plants. J. Biol. Chem. 275: 2276-2280 [Abstract] [Full Text]  
• Wendt, K. U., Poralla, K., Schulz, G. E. (1997). Structure and Function of a Squalene Cyclase. Science 277: 1811-1815 [Abstract] [Full Text]  
• Vogel, B. S., Wildung, M. R., Vogel, G., Croteau, R. (1996). Abietadiene Synthase from Grand Fir (Abies grandis). cDNA ISOLATION, CHARACTERIZATION, AND BACTERIAL EXPRESSION OF A BIFUNCTIONAL DITERPENE CYCLASE INVOLVED IN RESIN ACID BIOSYNTHESIS. J. Biol. Chem. 271: 23262-23268 [Abstract] [Full Text]