This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heffernan, E J Articles by Guiney, D Search for Related Content PubMed PubMed Citation Articles by Heffernan, E J Articles by Guiney, D

research-article

The Salmonella typhimurium virulence plasmid complement resistance gene rck is homologous to a family of virulence-related outer membrane protein genes, including pagC and ail.

Department of Medicine, University of California, San Diego 92103.

ABSTRACT

A fragment of the Salmonella typhimurium virulence plasmid containing the rck locus, when cloned in the recombinant cosmid pADE016, was shown previously to confer high-level complement resistance on both rough and smooth Escherichia coli, Salmonella minnesota, and S. typhimurium and was associated with the production of an outer membrane protein. We determined the nucleotide sequence of the fragment containing the rck locus. Mutations in the two major open reading frames confirmed that the complement resistance mediated by pADE016 was due to a single 555-bp rck gene encoding a 17-kDa outer membrane protein. Analysis of the rck gene revealed that the Rck outer membrane protein consisted of 185 amino acid residues, with a calculated postcleavage molecular mass of 17.4 kDa. Rck is homologous to a family of outer membrane proteins expressed in gram-negative bacteria, two of which have been associated with virulence-related phenotypes: PagC, required by S. typhimurium for survival in macrophages and for virulence in mice; and Ail, a product of the Yersinia enterocolitica chromosome capable of mediating bacterial adherence to and invasion of epithelial cell lines. Rck, most closely related to PagC, represents the third outer membrane protein in this five-member family with a distinct virulence-associated phenotype.

This article has been cited by other articles:

• Kolodziejek, A. M., Sinclair, D. J., Seo, K. S., Schnider, D. R., Deobald, C. F., Rohde, H. N., Viall, A. K., Minnich, S. S., Hovde, C. J., Minnich, S. A., Bohach, G. A. (2007). Phenotypic characterization of OmpX, an Ail homologue of Yersinia pestis KIM. Microbiology 153: 2941-2951 [Abstract] [Full Text]  
• Nishio, M., Okada, N., Miki, T., Haneda, T., Danbara, H. (2005). Identification of the outer-membrane protein PagC required for the serum resistance phenotype in Salmonella enterica serovar Choleraesuis. Microbiology 151: 863-873 [Abstract] [Full Text]  
• Otto, K., Hermansson, M. (2004). Inactivation of ompX Causes Increased Interactions of Type 1 Fimbriated Escherichia coli with Abiotic Surfaces. J. Bacteriol. 186: 226-234 [Abstract] [Full Text]  
• Takaya, A., Suzuki, M., Matsui, H., Tomoyasu, T., Sashinami, H., Nakane, A., Yamamoto, T. (2003). Lon, a Stress-Induced ATP-Dependent Protease, Is Critically Important for Systemic Salmonella enterica Serovar Typhimurium Infection of Mice. Infect. Immun. 71: 690-696 [Abstract] [Full Text]  
• Ho, T. D., Figueroa-Bossi, N., Wang, M., Uzzau, S., Bossi, L., Slauch, J. M. (2002). Identification of GtgE, a Novel Virulence Factor Encoded on the Gifsy-2 Bacteriophage of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 184: 5234-5239 [Abstract] [Full Text]  
• Brodsky, I. E., Ernst, R. K., Miller, S. I., Falkow, S. (2002). mig-14 Is a Salmonella Gene That Plays a Role in Bacterial Resistance to Antimicrobial Peptides. J. Bacteriol. 184: 3203-3213 [Abstract] [Full Text]  
• Deng, W., Li, Y., Vallance, B. A., Finlay, B. B. (2001). Locus of Enterocyte Effacement from Citrobacter rodentium: Sequence Analysis and Evidence for Horizontal Transfer among Attaching and Effacing Pathogens. Infect. Immun. 69: 6323-6335 [Abstract] [Full Text]  
• Haneda, T., Okada, N., Nakazawa, N., Kawakami, T., Danbara, H. (2001). Complete DNA Sequence and Comparative Analysis of the 50-Kilobase Virulence Plasmid of Salmonella enterica Serovar Choleraesuis. Infect. Immun. 69: 2612-2620 [Abstract] [Full Text]  
• Toguchi, A., Siano, M., Burkart, M., Harshey, R. M. (2000). Genetics of Swarming Motility in Salmonella enterica Serovar Typhimurium: Critical Role for Lipopolysaccharide. J. Bacteriol. 182: 6308-6321 [Abstract] [Full Text]  
• Schnabel, E. L., Jones, A. L. (1999). Distribution of Tetracycline Resistance Genes and Transposons among Phylloplane Bacteria in Michigan Apple Orchards. Appl. Environ. Microbiol. 65: 4898-4907 [Abstract] [Full Text]  
• Darwin, K. H., Miller, V. L. (1999). Molecular Basis of the Interaction of Salmonella with the Intestinal Mucosa. Clin. Microbiol. Rev. 12: 405-428 [Abstract] [Full Text]  
• Chu, C., Hong, S.-F., Tsai, C., Lin, W.-S., Liu, T.-P., Ou, J. T. (1999). Comparative Physical and Genetic Maps of the Virulence Plasmids of Salmonella enterica Serovars Typhimurium, Enteritidis, Choleraesuis, and Dublin. Infect. Immun. 67: 2611-2614 [Abstract] [Full Text]  
• Boyd, E. F., Hartl, D. L. (1998). Salmonella Virulence Plasmid: Modular Acquisition of the spv Virulence Region by an F-Plasmid in Salmonella enterica Subspecies I and Insertion Into the Chromosome of Subspecies II, IIIa, IV and VII Isolates. Genetics 149: 1183-1190 [Abstract] [Full Text]  
• Ahmer, B. M. M., van Reeuwijk, J., Timmers, C. D., Valentine, P. J., Heffron, F. (1998). Salmonella typhimurium Encodes an SdiA Homolog, a Putative Quorum Sensor of the LuxR Family, That Regulates Genes on the Virulence Plasmid. J. Bacteriol. 180: 1185-1193 [Abstract] [Full Text]