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Influence of attractants and repellents on methyl group turnover on methyl-accepting chemotaxis proteins of Bacillus subtilis and role of CheW.

Department of Biochemistry, College of Medicine, University of Illinois, Urbana 61801.

ABSTRACT

The ability of attractants and repellents to affect the turnover of methyl groups on the methyl-accepting chemotaxis proteins (MCPs) was examined for Bacillus subtilis. Attractants were found to cause an increase in the turnover of methyl groups esterified to the MCPs, while repellents caused a decrease. These reactions do not require CheW. However, a cheW null mutant exhibits enhanced turnover in unstimulated cells. Assuming that the turnover of methyl groups on the MCPs reflects a change in the activity of CheA, these results suggest that the activation of CheA via chemoeffector binding at the receptor does not require CheW.

This article has been cited by other articles:

• Kirby, J. R., Saulmon, M. M., Kristich, C. J., Ordal, G. W. (1999). CheY-dependent Methylation of the Asparagine Receptor, McpB, during Chemotaxis in Bacillus subtilis. J. Biol. Chem. 274: 11092-11100 [Abstract] [Full Text]