This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Krüger, N Articles by Steinbüchel, A Search for Related Content PubMed PubMed Citation Articles by Krüger, N Articles by Steinbüchel, A

research-article

Identification of acoR, a regulatory gene for the expression of genes essential for acetoin catabolism in Alcaligenes eutrophus H16.

Institut für Mikrobiologie, Georg-August-Universität zu Göttingen, Germany.

ABSTRACT

Two hundred thirty-nine base pairs upstream from acoXABC, which encodes the Alcaligenes eutrophus H16 structural genes essential for cleavage of acetoin, the 2,004-bp acoR gene was identified. acoR encodes a protein of 668 amino acids with a molecular mass of 72.9 kDa. The amino acid sequence deduced from acoR exhibited homologies to the primary structures of transcriptional activators such as NifA of Azotobacter vinelandii, NtrC of Klebsiella pneumoniae, and HoxA of A. eutrophus. Striking similarities to the central domain of these proteins and the presence of a typical nucleotide-binding site (GETGSGK) as well as of a C-terminal helix-turn-helix motif as a DNA-binding site were revealed. Between acoR and acoXABC, two different types of sequences with dual rotational symmetry [CAC-(N11 to N18)-GTG and TGT-(N10 to N14)-ACA] were found; these sequences are similar to NtrC and NifA upstream activator sequences, respectively. Determination of the N-terminal amino acid sequence of an acoR'-'lacZ gene fusion identified the translational start of acoR. S1 nuclease protection assay identified the transcriptional start site 109 bp upstream of acoR. The promoter region (TTGCGC-N18-TACATT) resembled the sigma 70 consensus sequence of Escherichia coli. Analysis of an acoR'-'lacZ fusion and primer extension studies revealed that acoR was expressed at a low level under all culture conditions, whereas acoXABC was expressed only in acetoin-grown cells. The insertions of Tn5 in six transposon-induced acetoin-negative mutants of A. eutrophus were mapped within acoR. On the basis of these studies, it is probable that AcoR represents a regulatory protein which is required for sigma 54-dependent transcription of acoXABC.

This article has been cited by other articles:

• Kurth, E. G., Doughty, D. M., Bottomley, P. J., Arp, D. J., Sayavedra-Soto, L. A. (2008). Involvement of BmoR and BmoG in n-alkane metabolism in 'Pseudomonas butanovora'. Microbiology 154: 139-147 [Abstract] [Full Text]  
• Jakobsen, J. S., Jelsbak, L., Jelsbak, L., Welch, R. D., Cummings, C., Goldman, B., Stark, E., Slater, S., Kaiser, D. (2004). {sigma}54 Enhancer Binding Proteins and Myxococcus xanthus Fruiting Body Development. J. Bacteriol. 186: 4361-4368 [Abstract] [Full Text]  
• Stafford, G. P., Scanlan, J., McDonald, I. R., Murrell, J. C. (2003). rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b. Microbiology 149: 1771-1784 [Abstract] [Full Text]  
• Sluis, M. K., Larsen, R. A., Krum, J. G., Anderson, R., Metcalf, W. W., Ensign, S. A. (2002). Biochemical, Molecular, and Genetic Analyses of the Acetone Carboxylases from Xanthobacter autotrophicus Strain Py2 and Rhodobacter capsulatus Strain B10. J. Bacteriol. 184: 2969-2977 [Abstract] [Full Text]  
• Bartosik, D., Baj, J., Bartosik, A. A., Wlodarczyk, M. (2002). Characterization of the replicator region of megaplasmid pTAV3 of Paracoccus versutus and search for plasmid-encoded traits. Microbiology 148: 871-881 [Abstract] [Full Text]  
• Gama-Castro, S., Nunez, C., Segura, D., Moreno, S., Guzman, J., Espin, G. (2001). Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by {sigma}54: Role in Alcohol Catabolism and Encystment. J. Bacteriol. 183: 6169-6174 [Abstract] [Full Text]  
• Schwartz, E., Buhrke, T., Gerischer, U., Friedrich, B. (1999). Positive Transcriptional Feedback Controls Hydrogenase Expression in Alcaligenes eutrophus H16. J. Bacteriol. 181: 5684-5692 [Abstract] [Full Text]  
• Debarbouille, M., Gardan, R., Arnaud, M., Rapoport, G. (1999). Role of BkdR, a Transcriptional Activator of the SigL-Dependent Isoleucine and Valine Degradation Pathway in Bacillus subtilis. J. Bacteriol. 181: 2059-2066 [Abstract] [Full Text]