This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Basu, J Articles by Chakrabarti, P Search for Related Content PubMed PubMed Citation Articles by Basu, J Articles by Chakrabarti, P

research-article

Purification and partial characterization of a penicillin-binding protein from Mycobacterium smegmatis.

Department of Chemistry, Bose Institute, Calcutta, India.

ABSTRACT

Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography of the solubilized proteins on ampicillin-linked CH Sepharose 4B and DE-52. The purified PBP (M(r), 49,500) catalyzed a model transpeptidase reaction with the tripeptide acetyl2-L-Lys-D-Ala-D-Ala as the substrate and Gly-Gly as the acceptor. The transpeptidase activity was inhibited by 50% at a benzylpenicillin concentration of 1.8 x 10(-7) M, which was similar to the concentration (1.1 x 10(-7) M) of benzylpenicillin required to saturate to 50% this PBP. Of several antibiotics tested, the concentration of antibiotic required to inhibit [35S]penicillin binding by 90% was found to be the lowest for cefoxitin and Sch 34343.

This article has been cited by other articles:

• Goffin, C., Ghuysen, J.-M. (2002). Biochemistry and Comparative Genomics of SxxK Superfamily Acyltransferases Offer a Clue to the Mycobacterial Paradox: Presence of Penicillin-Susceptible Target Proteins versus Lack of Efficiency of Penicillin as Therapeutic Agent. Microbiol. Mol. Biol. Rev. 66: 702-738 [Abstract] [Full Text]  
• Rao, M., Streur, T. L., Aldwell, F. E., Cook, G. M. (2001). Intracellular pH regulation by Mycobacterium smegmatis and Mycobacterium bovis BCG. Microbiology 147: 1017-1024 [Abstract] [Full Text]  
• Billman-Jacobe, H., Haites, R. E., Coppel, R. L. (1999). Characterization of a Mycobacterium smegmatis Mutant Lacking Penicillin Binding Protein 1. Antimicrob. Agents Chemother. 43: 3011-3013 [Abstract] [Full Text]