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research-article

Production of an extracellular milk-clotting activity during development in Myxococcus xanthus.

Equipe de Microbiologie, Faculté des Sciences et Techniques, Centre National de la Recherche Scientifique URA 203, Mount-Saint-Aignan, France.

ABSTRACT

We describe here an extracellular proteolytic activity secreted during both growth and submerged development by Myxococcus xanthus DK1622. This activity yields the clotting of kappa-casein at pH 6 and is inhibited by specific inhibitors of aspartic proteases. Secretion of this milk-clotting proteolytic activity (of Mcp) is time regulated during the developmental cycle, with a large increase near 9 h poststarvation, but its production does not require cell-cell contact. The lack of secretion of this activity by several developmental mutants in submerged development conditions shows that Mcp production is developmentally regulated.

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