This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Duwat, P Articles by Gruss, A Search for Related Content PubMed PubMed Citation Articles by Duwat, P Articles by Gruss, A

 Previous Article  |  Next Article 

J Bacteriol. 1992 August; 174(15): 5171-5175

research-article

A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction.

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

ABSTRACT

An internal fragment of the recA gene from eight gram-positive organisms has been amplified by using degenerate primers in a polymerase chain reaction. The internal 348- or 360-bp recA DNA segments from Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus bulgaricus, Lactobacillus helveticus, Leuconostoc mesanteroides, Listeria monocytogenes, Staphylococcus aureus, and Streptococcus salivarus subsp. thermophilus were amplified, cloned, and sequenced. The G + C contents of the DNA from these species range from 28 to 52%. The sequences of the bacterial recA genes show strong relatedness. This method is particularly useful for the recovery of the recA genes of gram-positive bacteria and avoids the difficulties of using a genetic complementation test for cloning.

This article has been cited by other articles:

• Nahrstedt, H., Schroder, C., Meinhardt, F. (2005). Evidence for two recA genes mediating DNA repair in Bacillus megaterium. Microbiology 151: 775-787 [Abstract] [Full Text]  
• Ze-Ze, L., Tenreiro, R., Paveia, H. (2000). The Oenococcus oeni genome: physical and genetic mapping of strain GM and comparison with the genome of a 'divergent' strain, PSU-1. Microbiology 146: 3195-3204 [Abstract] [Full Text]  
• van Waasbergen, L. G., Balkwill, D. L., Crocker, F. H., Bjornstad, B. N., Miller, R. V. (2000). Genetic Diversity among Arthrobacter Species Collected across a Heterogeneous Series of Terrestrial Deep-Subsurface Sediments as Determined on the Basis of 16S rRNA and recA Gene Sequences. Appl. Environ. Microbiol. 66: 3454-3463 [Abstract] [Full Text]  
• Blackwood, K. S., He, C., Gunton, J., Turenne, C. Y., Wolfe, J., Kabani, A. M. (2000). Evaluation of recA Sequences for Identification of Mycobacterium Species. J. Clin. Microbiol. 38: 2846-2852 [Abstract] [Full Text]  
• Froeliger, E. H., Tomich, M., Fives-Taylor, P. (1999). Construction and Analysis of a Streptococcus parasanguis recA Mutant: Homologous Recombination Is Not Required for Adhesion in an In Vitro Tooth Surface Model. J. Bacteriol. 181: 63-67 [Abstract] [Full Text]