This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lottenberg, R Articles by Curtiss, R Search for Related Content PubMed PubMed Citation Articles by Lottenberg, R Articles by Curtiss, R, 3rd

research-article

Cloning, sequence analysis, and expression in Escherichia coli of a streptococcal plasmin receptor.

Department of Medicine, University of Florida, Gainesville 32610.

ABSTRACT

Plasmin(ogen) receptors are expressed by many gram-positive and gram-negative bacteria. We previously isolated a plasmin receptor from a pathogenic group A streptococcal strain (C. C. Broder, R. Lottenberg, G. O. von Mering, K. H. Johnston, and M. D. P. Boyle, J. Biol. Chem. 266:4922-4928, 1991). The gene encoding this plasmin receptor, plr, was isolated from a lambda gt11 library of chromosomal DNA from group A streptococcal strain 64/14 by screening plaques with antibodies raised against the purified streptococcal plasmin receptor protein. The gene was subcloned by using a low-copy-number plasmid and stably expressed in Escherichia coli, resulting in the production of an immunoreactive and functional receptor protein. The DNA sequence of the gene contained an open reading frame encoding 335 amino acids with a predicted molecular weight of 35,787. Upstream of the open reading frame, putative promoter and ribosomal binding site sequences were identified. The experimentally derived amino acid sequences of the N terminus and three cyanogen bromide fragments of the purified streptococcal plasmin receptor protein corresponded to the predicted sequence encoded by plr. The deduced amino acid sequence for the plasmin receptor protein revealed significant similarity (39 to 54% identical amino acid residues) to glyceraldehyde 3-phosphate dehydrogenases.

This article has been cited by other articles:

• Antikainen, J., Kupannen, V., Lahteenmaki, K., Korhonen, T. K. (2007). pH-Dependent Association of Enolase and Glyceraldehyde-3-Phosphate Dehydrogenase of Lactobacillus crispatus with the Cell Wall and Lipoteichoic Acids. J. Bacteriol. 189: 4539-4543 [Abstract] [Full Text]  
• Cole, J. N., McArthur, J. D., McKay, F. C., Sanderson-Smith, M. L., Cork, A. J., Ranson, M., Rohde, M., Itzek, A., Sun, H., Ginsburg, D., Kotb, M., Nizet, V., Chhatwal, G. S., Walker, M. J. (2006). Trigger for group A streptococcal M1T1 invasive disease. FASEB J. 20: 1745-1747 [Abstract] [Full Text]  
• Terao, Y., Yamaguchi, M., Hamada, S., Kawabata, S. (2006). Multifunctional Glyceraldehyde-3-phosphate Dehydrogenase of Streptococcus pyogenes Is Essential for Evasion from Neutrophils. J. Biol. Chem. 281: 14215-14223 [Abstract] [Full Text]  
• Sanderson-Smith, M., Batzloff, M., Sriprakash, K. S., Dowton, M., Ranson, M., Walker, M. J. (2006). Divergence in the Plasminogen-binding Group A Streptococcal M Protein Family: FUNCTIONAL CONSERVATION OF BINDING SITE AND POTENTIAL ROLE FOR IMMUNE SELECTION OF VARIANTS. J. Biol. Chem. 281: 3217-3226 [Abstract] [Full Text]  
• Boel, G., Jin, H., Pancholi, V. (2005). Inhibition of Cell Surface Export of Group A Streptococcal Anchorless Surface Dehydrogenase Affects Bacterial Adherence and Antiphagocytic Properties. Infect. Immun. 73: 6237-6248 [Abstract] [Full Text]  
• Yoshizawa, N., Yamakami, K., Fujino, M., Oda, T., Tamura, K., Matsumoto, K., Sugisaki, T., Boyle, M. D.P. (2004). Nephritis-Associated Plasmin Receptor and Acute Poststreptococcal Glomerulonephritis: Characterization of the Antigen and Associated Immune Response. J. Am. Soc. Nephrol. 15: 1785-1793 [Abstract] [Full Text]  
• Bergmann, S., Rohde, M., Hammerschmidt, S. (2004). Glyceraldehyde-3-Phosphate Dehydrogenase of Streptococcus pneumoniae Is a Surface-Displayed Plasminogen-Binding Protein. Infect. Immun. 72: 2416-2419 [Abstract] [Full Text]  
• Derbise, A., Song, Y. P., Parikh, S., Fischetti, V. A., Pancholi, V. (2004). Role of the C-Terminal Lysine Residues of Streptococcal Surface Enolase in Glu- and Lys-Plasminogen-Binding Activities of Group A Streptococci. Infect. Immun. 72: 94-105 [Abstract] [Full Text]  
• Kalia, A., Bessen, D. E. (2004). Natural Selection and Evolution of Streptococcal Virulence Genes Involved in Tissue-Specific Adaptations. J. Bacteriol. 186: 110-121 [Abstract] [Full Text]  
• Lu, H.-Z., Weng, X.-H., Zhu, B., Li, H., Yin, Y.-K., Zhang, Y.-X., Haas, D. W., Tang, Y.-W. (2003). Major Outbreak of Toxic Shock-Like Syndrome Caused by Streptococcus mitis. J. Clin. Microbiol. 41: 3051-3055 [Abstract] [Full Text]  
• GRIFANTINI, R., BARTOLINI, E., MUZZI, A., DRAGHI, M., FRIGIMELICA, E., BERGER, J., RANDAZZO, F., GRANDI, G. (2002). Gene Expression Profile in Neisseria meningitidis and Neisseria lactamica upon Host-Cell Contact: From Basic Research to Vaccine Development. Ann. N. Y. Acad. Sci. 975: 202-216 [Abstract] [Full Text]  
• Svensson, M. D., Sjobring, U., Luo, F., Bessen, D. E. (2002). Roles of the plasminogen activator streptokinase and the plasminogen-associated M protein in an experimental model for streptococcal impetigo. Microbiology 148: 3933-3945 [Abstract] [Full Text]  
• Ajdic', D., McShan, W. M., McLaughlin, R. E., Savic', G., Chang, J., Carson, M. B., Primeaux, C., Tian, R., Kenton, S., Jia, H., Lin, S., Qian, Y., Li, S., Zhu, H., Najar, F., Lai, H., White, J., Roe, B. A., Ferretti, J. J. (2002). Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc. Natl. Acad. Sci. USA 99: 14434-14439 [Abstract] [Full Text]  
• WHITING, G.C., EVANS, J.T., PATEL, S., GILLESPIE, S.H. (2002). Purification of native {alpha}-enolase from Streptococcus pneumoniae that binds plasminogen and is immunogenic. J Med Microbiol 51: 837-843 [Abstract] [Full Text]  
• Caballero, A. R., Lottenberg, R., Johnston, K. H. (1999). Cloning, Expression, Sequence Analysis, and Characterization of Streptokinases Secreted by Porcine and Equine Isolates of Streptococcus equisimilis. Infect. Immun. 67: 6478-6486 [Abstract] [Full Text]  
• Svensson, M. D., Sjobring, U., Bessen, D. E. (1999). Selective Distribution of a High-Affinity Plasminogen-Binding Site among Group A Streptococci Associated with Impetigo. Infect. Immun. 67: 3915-3920 [Abstract] [Full Text]  
• Modun, B., Williams, P. (1999). The Staphylococcal Transferrin-Binding Protein Is a Cell Wall Glyceraldehyde-3-Phosphate Dehydrogenase. Infect. Immun. 67: 1086-1092 [Abstract] [Full Text]  
• Kukkonen, M., Saarela, S., Lahteenmaki, K., Hynonen, U., Westerlund-Wikstrom, B., Rhen, M., Korhonen, T. K. (1998). Identification of Two Laminin-Binding Fimbriae, the Type 1 Fimbria of Salmonella enterica Serovar Typhimurium and the G Fimbria of Escherichia coli, as Plasminogen Receptors. Infect. Immun. 66: 4965-4970 [Abstract] [Full Text]  
• Pantzar, M., Ljungh, A., Wadstrom, T. (1998). Plasminogen Binding and Activation at the Surface of Helicobacter pylori CCUG 17874. Infect. Immun. 66: 4976-4980 [Abstract] [Full Text]  
• Goto, H., Kawaoka, Y. (1998). A novel mechanism for the acquisition of virulence by a human influenza A virus. Proc. Natl. Acad. Sci. USA 95: 10224-10228 [Abstract] [Full Text]  
• Bowe, F., Lipps, C. J., Tsolis, R. M., Groisman, E., Heffron, F., Kusters, J. G. (1998). At Least Four Percent of the Salmonella typhimurium Genome Is Required for Fatal Infection of Mice. Infect. Immun. 66: 3372-3377 [Abstract] [Full Text]  
• Pancholi, V., Fischetti, V. A. (1998). alpha -Enolase, a Novel Strong Plasmin(ogen) Binding Protein on the Surface of Pathogenic Streptococci. J. Biol. Chem. 273: 14503-14515 [Abstract] [Full Text]  
• Ringdahl, U., Svensson, M., Wistedt, A. C., Renne, T., Kellner, R., Muller-Esterl, W., Sjobring, U. (1998). Molecular Co-operation between Protein PAM and Streptokinase for Plasmin Acquisition by Streptococcus pyogenes. J. Biol. Chem. 273: 6424-6430 [Abstract] [Full Text]  
• Park, P. W., Rosenbloom, J., Abrams, W. R., Rosenbloom, J., Mecham, R. P. (1996). Molecular Cloning and Expression of the Gene for Elastin-binding Protein (ebpS) in Staphylococcus aureus. J. Biol. Chem. 271: 15803-15809 [Abstract] [Full Text]