This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de Vries, G E Articles by Dijkhuizen, L Search for Related Content PubMed PubMed Citation Articles by de Vries, G E Articles by Dijkhuizen, L

research-article

Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene.

Department of Microbiology, University of Groningen, Haren, The Netherlands.

ABSTRACT

The gene (mdh) coding for methanol dehydrogenase (MDH) of thermotolerant, methylotroph Bacillus methanolicus C1 has been cloned and sequenced. The deduced amino acid sequence of the mdh gene exhibited similarity to those of five other alcohol dehydrogenase (type III) enzymes, which are distinct from the long-chain zinc-containing (type I) or short-chain zinc-lacking (type II) enzymes. Highly efficient expression of the mdh gene in Escherichia coli was probably driven from its own promoter sequence. After purification of MDH from E. coli, the kinetic and biochemical properties of the enzyme were investigated. The physiological effect of MDH synthesis in E. coli and the role of conserved sequence patterns in type III alcohol dehydrogenases have been analyzed and are discussed.

This article has been cited by other articles:

• Haveman, S. A., DiDonato, R. J. Jr., Villanueva, L., Shelobolina, E. S., Postier, B. L., Xu, B., Liu, A., Lovley, D. R. (2008). Genome-Wide Gene Expression Patterns and Growth Requirements Suggest that Pelobacter carbinolicus Reduces Fe(III) Indirectly via Sulfide Production. Appl. Environ. Microbiol. 74: 4277-4284 [Abstract] [Full Text]  
• Ohhata, N., Yoshida, N., Egami, H., Katsuragi, T., Tani, Y., Takagi, H. (2007). An Extremely Oligotrophic Bacterium, Rhodococcus erythropolis N9T-4, Isolated from Crude Oil. J. Bacteriol. 189: 6824-6831 [Abstract] [Full Text]  
• Jakobsen, O. M., Benichou, A., Flickinger, M. C., Valla, S., Ellingsen, T. E., Brautaset, T. (2006). Upregulated Transcription of Plasmid and Chromosomal Ribulose Monophosphate Pathway Genes Is Critical for Methanol Assimilation Rate and Methanol Tolerance in the Methylotrophic Bacterium Bacillus methanolicus.. J. Bacteriol. 188: 3063-3072 [Abstract] [Full Text]  
• Brautaset, T., Jakobsen, O. M., Flickinger, M. C., Valla, S., Ellingsen, T. E. (2004). Plasmid-Dependent Methylotrophy in Thermotolerant Bacillus methanolicus. J. Bacteriol. 186: 1229-1238 [Abstract] [Full Text]  
• Brautaset, T., Williams, M. D., Dillingham, R. D., Kaufmann, C., Bennaars, A., Crabbe, E., Flickinger, M. C. (2003). Role of the Bacillus methanolicus Citrate Synthase II Gene, citY, in Regulating the Secretion of Glutamate in L-Lysine-Secreting Mutants. Appl. Environ. Microbiol. 69: 3986-3995 [Abstract] [Full Text]  
• Raynaud, C., Sarcabal, P., Meynial-Salles, I., Croux, C., Soucaille, P. (2003). Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum. Proc. Natl. Acad. Sci. USA 100: 5010-5015 [Abstract] [Full Text]  
• Daiyasu, H., Hiroike, T., Koga, Y., Toh, H. (2002). Analysis of membrane stereochemistry with homology modeling of sn-glycerol-1-phosphate dehydrogenase. Protein Eng Des Sel 15: 987-995 [Abstract] [Full Text]  
• Hektor, H. J., Kloosterman, H., Dijkhuizen, L. (2002). Identification of a Magnesium-dependent NAD(P)(H)-binding Domain in the Nicotinoprotein Methanol Dehydrogenase from Bacillus methanolicus. J. Biol. Chem. 277: 46966-46973 [Abstract] [Full Text]  
• Pluschkell, S. B., Flickinger, M. C. (2002). Dissimilation of [13C]methanol by continuous cultures of Bacillus methanolicus MGA3 at 50 {degrees}C studied by 13C NMR and isotope-ratio mass spectrometry. Microbiology 148: 3223-3233 [Abstract] [Full Text]  
• Kloosterman, H., Vrijbloed, J. W., Dijkhuizen, L. (2002). Molecular, Biochemical, and Functional Characterization of a Nudix Hydrolase Protein That Stimulates the Activity of a Nicotinoprotein Alcohol Dehydrogenase. J. Biol. Chem. 277: 34785-34792 [Abstract] [Full Text]  
• Fontaine, L., Meynial-Salles, I., Girbal, L., Yang, X., Croux, C., Soucaille, P. (2002). Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824. J. Bacteriol. 184: 821-830 [Abstract] [Full Text]  
• Yasueda, H., Kawahara, Y., Sugimoto, S.-i. (1999). Bacillus subtilis yckG and yckF Encode Two Key Enzymes of the Ribulose Monophosphate Pathway Used by Methylotrophs, and yckH Is Required for Their Expression. J. Bacteriol. 181: 7154-7160 [Abstract] [Full Text]  
• Lu, Z., Cabiscol, E., Obradors, N., Tamarit, J., Ros, J., Aguilar, J., Lin, E. C. C. (1998). Evolution of an Escherichia coli Protein with Increased Resistance to Oxidative Stress. J. Biol. Chem. 273: 8308-8316 [Abstract] [Full Text]  
• Nakamura, A., Amikura, R., Mukai, M., Kobayashi, S., Lasko, P. F. (1996). Requirement for a Noncoding RNA in Drosophila Polar Granules for Germ Cell Establishment. Science 274: 2075-2079 [Abstract] [Full Text]