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research-article

Efficiency of homologous intermolecular recombination at different locations on the Bacillus subtilis chromosome.

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

ABSTRACT

The efficiencies of intermolecular recombination at 12 different locations on the Bacillus subtilis chromosome were determined by transforming competent cells with a nonreplicative plasmid. The efficiencies varied by only about threefold but were significantly different (P less than 0.05 by a chi-square test) for approximately 20% of the locations. The recA gene product is required for recombination, and the addA gene product appears to affect the variation in a site-specific way.

This article has been cited by other articles:

• Itaya, M. (1999). Genetic Transfer of Large DNA Inserts to Designated Loci of the Bacillus subtilis 168 Genome. J. Bacteriol. 181: 1045-1048 [Abstract] [Full Text]