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DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli.

Department of Biochemistry, University of Wisconsin, Madison 53706.

ABSTRACT

The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli. Cells deleted for dnaK or dnaJ, or with some mutations in the dnaK or grpE gene, are nonmotile, lack flagella, exhibit a 10- to 20-fold decrease in the rate of synthesis of flagellin, and show reduced rates of transcription of both the flhD master operon (encoding FlhD and FlhC) and the fliA operon (encoding sigma F). Genetic studies suggest that DnaK and DnaJ define a regulatory pathway affecting flhD and fliA synthesis that is independent of cyclic AMP-catabolite gene activator protein or the chemotaxis system.

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• Stewart, B. J., McCarter, L. L. (2003). Lateral Flagellar Gene System of Vibrio parahaemolyticus. J. Bacteriol. 185: 4508-4518 [Abstract] [Full Text]  
• Yona-Nadler, C., Umanski, T., Aizawa, S.-I., Friedberg, D., Rosenshine, I. (2003). Integration host factor (IHF) mediates repression of flagella in enteropathogenic and enterohaemorrhagic Escherichia coli. Microbiology 149: 877-884 [Abstract] [Full Text]  
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• Genevaux, P., Schwager, F., Georgopoulos, C., Kelley, W. L. (2001). The djlA Gene Acts Synergistically with dnaJ in Promoting Escherichia coli Growth. J. Bacteriol. 183: 5747-5750 [Abstract] [Full Text]  
• Chilcott, G. S., Hughes, K. T. (2000). Coupling of Flagellar Gene Expression to Flagellar Assembly in Salmonella enterica Serovar Typhimurium and Escherichia coli. Microbiol. Mol. Biol. Rev. 64: 694-708 [Abstract] [Full Text]  
• Soutourina, O., Kolb, A., Krin, E., Laurent-Winter, C., Rimsky, S., Danchin, A., Bertin, P. (1999). Multiple Control of Flagellum Biosynthesis in Escherichia coli: Role of H-NS Protein and the Cyclic AMP-Catabolite Activator Protein Complex in Transcription of the flhDC Master Operon. J. Bacteriol. 181: 7500-7508 [Abstract] [Full Text]  
• Eberl, L., Molin, S., Givskov, M. (1999). Surface Motility of Serratia liquefaciens MG1. J. Bacteriol. 181: 1703-1712 [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Givskov, M., Östling, J., Eberl, L., Lindum, P. W., Christensen, A. B., Christiansen, G., Molin, S., Kjelleberg, S. (1998). Two Separate Regulatory Systems Participate in Control of Swarming Motility of Serratia liquefaciens MG1. J. Bacteriol. 180: 742-745 [Abstract] [Full Text]  
• Yang, Z., Geng, Y., Shi, W. (1998). A DnaK Homolog in Myxococcus xanthus Is Involved in Social Motility and Fruiting Body Formation. J. Bacteriol. 180: 218-224 [Abstract] [Full Text]  
• Crouy-Chanel, A. d., Kohiyama, M., Richarme, G. (1996). Specificity of DnaK for Arginine/Lysine and Effect of DnaJ on the Amino Acid Specificity of DnaK. J. Biol. Chem. 271: 15486-15490 [Abstract] [Full Text]  
• Karzai, A. W., McMacken, R. (1996). A Bipartite Signaling Mechanism Involved in DnaJ-mediated Activation of the Escherichia coli DnaK Protein. J. Biol. Chem. 271: 11236-11246 [Abstract] [Full Text]  
• Tsai, J., Douglas, M. G. (1996). A Conserved HPD Sequence of the J-domain Is Necessary for YDJ1 Stimulation of Hsp70 ATPase Activity at a Site Distinct from Substrate Binding. J. Biol. Chem. 271: 9347-9354 [Abstract] [Full Text]  
• Kamath-Loeb, A. S., Lu, C. Z., Suh, W.-C., Lonetto, M. A., Gross, C. A. (1995). Analysis of Three DnaK Mutant Proteins Suggests That Progression through the ATPase Cycle Requires Conformational Changes. J. Biol. Chem. 270: 30051-30059 [Abstract] [Full Text]