This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Groisman, E A Articles by Solomon, F Search for Related Content PubMed PubMed Citation Articles by Groisman, E A Articles by Solomon, F

research-article

Molecular genetic analysis of the Escherichia coli phoP locus.

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-1093.

ABSTRACT

We have cloned the Escherichia coli phoP gene, a member of the family of environmentally responsive two-component systems, and found its deduced amino acid sequence to be 93% identical to that of the Salmonella typhimurium homolog, which encodes a major virulence regulator necessary for intramacrophage survival and resistance to cationic peptides of phagocytic cells. The phoP gene was mapped to kilobase 1202 on the Kohara map (25-min region) of the E. coli genome (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987) and found to be transcribed in a counterclockwise direction. Both E. coli and S. typhimurium phoP mutants were more sensitive than their isogenic wild-type strains to the frog-derived antibacterial peptide magainin 2, suggesting a role for PhoP in the response to various stresses in both enteric species.

This article has been cited by other articles:

• Purdy, G. E., Fisher, C. R., Payne, S. M. (2007). IcsA Surface Presentation in Shigella flexneri Requires the Periplasmic Chaperones DegP, Skp, and SurA. J. Bacteriol. 189: 5566-5573 [Abstract] [Full Text]  
• Calvez, S., Rince, A., Auffray, Y., Prevost, H., Drider, D. (2007). Identification of new genes associated with intermediate resistance of Enterococcus faecalis to divercin V41, a pediocin-like bacteriocin. Microbiology 153: 1609-1618 [Abstract] [Full Text]  
• Zwir, I., Shin, D., Kato, A., Nishino, K., Latifi, T., Solomon, F., Hare, J. M., Huang, H., Groisman, E. A. (2005). Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica. Proc. Natl. Acad. Sci. USA 102: 2862-2867 [Abstract] [Full Text]  
• Jia, W., Zoeiby, A. E., Petruzziello, T. N., Jayabalasingham, B., Seyedirashti, S., Bishop, R. E. (2004). Lipid Trafficking Controls Endotoxin Acylation in Outer Membranes of Escherichia coli. J. Biol. Chem. 279: 44966-44975 [Abstract] [Full Text]  
• Lee, H., Hsu, F.-F., Turk, J., Groisman, E. A. (2004). The PmrA-Regulated pmrC Gene Mediates Phosphoethanolamine Modification of Lipid A and Polymyxin Resistance in Salmonella enterica. J. Bacteriol. 186: 4124-4133 [Abstract] [Full Text]  
• Lejona, S., Aguirre, A., Cabeza, M. L., Vescovi, E. G., Soncini, F. C. (2003). Molecular Characterization of the Mg2+-Responsive PhoP-PhoQ Regulon in Salmonella enterica. J. Bacteriol. 185: 6287-6294 [Abstract] [Full Text]  
• Minagawa, S., Ogasawara, H., Kato, A., Yamamoto, K., Eguchi, Y., Oshima, T., Mori, H., Ishihama, A., Utsumi, R. (2003). Identification and Molecular Characterization of the Mg2+ Stimulon of Escherichia coli. J. Bacteriol. 185: 3696-3702 [Abstract] [Full Text]  
• Hong, R. W., Shchepetov, M., Weiser, J. N., Axelsen, P. H. (2003). Transcriptional Profile of the Escherichia coli Response to the Antimicrobial Insect Peptide Cecropin A. Antimicrob. Agents Chemother. 47: 1-6 [Abstract] [Full Text]  
• Bochner, B. R., Gadzinski, P., Panomitros, E. (2001). Phenotype MicroArrays for High-Throughput Phenotypic Testing and Assay of Gene Function. Genome Res. 11: 1246-1255 [Abstract] [Full Text]  
• Groisman, E. A. (2001). The Pleiotropic Two-Component Regulatory System PhoP-PhoQ. J. Bacteriol. 183: 1835-1842 [Full Text]  
• Kato, A., Tanabe, H., Utsumi, R. (1999). Molecular Characterization of the PhoP-PhoQ Two-Component System in Escherichia coli K-12: Identification of Extracellular Mg2+-Responsive Promoters. J. Bacteriol. 181: 5516-5520 [Abstract] [Full Text]  
• Turner, J., Cho, Y., Dinh, N.-N., Waring, A. J., Lehrer, R. I. (1998). Activities of LL-37, a Cathelin-Associated Antimicrobial Peptide of Human Neutrophils. Antimicrob. Agents Chemother. 42: 2206-2214 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Hall, B. G. (1998). Adaptive Mutagenesis at ebgR Is Regulated by PhoPQ. J. Bacteriol. 180: 2862-2865 [Abstract] [Full Text]