This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iost, I Articles by Dreyfus, M Search for Related Content PubMed PubMed Citation Articles by Iost, I Articles by Dreyfus, M

 Previous Article  |  Next Article 

J Bacteriol. 1992 January; 174(2): 619-622

research-article

Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo.

Laboratoire de Génétique Moléculaire (Centre National de la Recherche Scientifique D1302), Ecole Normale Supérieure, Paris, France.

ABSTRACT

We show that in Escherichia coli at 32 degrees C, the T7 RNA polymerase travels over the lacZ gene about eightfold faster than ribosomes travel over the corresponding mRNA. We discuss how the T7 phage might exploit this high rate in its growth optimization strategy and how it obviates the possible drawbacks of uncoupling transcription from translation.

This article has been cited by other articles:

• Wagner, S., Klepsch, M. M., Schlegel, S., Appel, A., Draheim, R., Tarry, M., Hogbom, M., van Wijk, K. J., Slotboom, D. J., Persson, J. O., de Gier, J.-W. (2008). Tuning Escherichia coli for membrane protein overexpression. Proc. Natl. Acad. Sci. USA 105: 14371-14376 [Abstract] [Full Text]  
• Paulus, M., Haslbeck, M., Watzele, M. (2004). RNA stem-loop enhanced expression of previously non-expressible genes. Nucleic Acids Res 32: e78-e78 [Abstract] [Full Text]  
• Serruto, D., Galeotti, C. L. (2004). The signal peptide sequence of a lytic transglycosylase of Neisseria meningitidis is involved in regulation of gene expression. Microbiology 150: 1427-1437 [Abstract] [Full Text]  
• Lopez, P. J., Marchand, I., Yarchuk, O., Dreyfus, M. (1998). Translation inhibitors stabilize Escherichia coli mRNAs independently of ribosome protection. Proc. Natl. Acad. Sci. USA 95: 6067-6072 [Abstract] [Full Text]  
• Aiso, T., Ohki, R. (1998). An rne-1 pnp-7 Double Mutation Suppresses the Temperature-Sensitive Defect of lacZ Gene Expression in a divE Mutant. J. Bacteriol. 180: 1389-1395 [Abstract] [Full Text]  
• Beletskii, A., Bhagwat, A. S. (1996). Transcription-induced mutations: Increase in C to T mutations in the nontranscribed strand during transcription in Escherichia coli. Proc. Natl. Acad. Sci. USA 93: 13919-13924 [Abstract] [Full Text]  
• Lee, K., Cohen, S. N. (2001). Effects of 3' Terminus Modifications on mRNA Functional Decay during in Vitro Protein Synthesis. J. Biol. Chem. 276: 23268-23274 [Abstract] [Full Text]