This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gadkari, D Articles by Meyer, O Search for Related Content PubMed PubMed Citation Articles by Gadkari, D Articles by Meyer, O

 Previous Article  |  Next Article 

J Bacteriol. 1992 November; 174(21): 6840-6843

research-article

Chemolithoautotrophic assimilation of dinitrogen by Streptomyces thermoautotrophicus UBT1: identification of an unusual N2-fixing system.

Lehrstuhl für Mikrobiologie, Universität Bayreuth, Germany.

ABSTRACT

Streptomyces thermoautotrophicus UBT1, which was isolated previously from a burning charcoal pile, was shown to utilize N2 as a sole nitrogen source when growing chemolithoautotrophically with CO or H2 plus CO2 under aerobic conditions at 65 degrees C. Doubling times under diazotrophic conditions were 10 h. S. thermoautotrophicus is a new CO- or H2-oxidizing, obligately chemolithoautotrophic, thermophilic, free-living, aerobic, N2-fixing streptomycete. Its ability to fix N2 was also evident from (i) the incorporation of substantial amounts of 15N2 (about 13%) into cell material, (ii) the formation of H2 during diazotrophic growth, (iii) the repression of 15N2 assimilation and H2 formation by ammonia, and (iv) culture growth yields with N2 as a nitrogen source that were significantly higher than those without any added nitrogen compounds (ca. 2.4 versus < 0.1 mg [dry weight]). The N2-fixing system of S. thermoautotrophicus exhibited several properties not apparent in the diazotrophic bacteria studied so far, since it was (i) incapable of reducing acetylene to ethylene or ethane and (ii) resistant to inhibition by acetylene or ethylene (5% [vol/vol] each), CO (40 to 70% [vol/vol]), or H2 (40% [vol/vol]). Under stringent conditions, nifH and nifDK gene probes from Klebsiella pneumoniae did not hybridize with total DNA from S. thermoautotrophicus.

This article has been cited by other articles:

• Xie, C.-H., Yokota, A. (2006). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil.. Int. J. Syst. Evol. Microbiol. 56: 1117-1121 [Abstract] [Full Text]  
• Hu, Y., Corbett, M. C., Fay, A. W., Webber, J. A., Hedman, B., Hodgson, K. O., Ribbe, M. W. (2005). Nitrogenase reactivity with P-cluster variants. Proc. Natl. Acad. Sci. USA 102: 13825-13830 [Abstract] [Full Text]  
• Ribbe, M., Gadkari, D., Meyer, O. (1997). N2 Fixation by Streptomyces thermoautotrophicus Involves a Molybdenum-Dinitrogenase and a Manganese-Superoxide Oxidoreductase That Couple N2 Reduction to the Oxidation of Superoxide Produced from O2 by a Molybdenum-CO Dehydrogenase. J. Biol. Chem. 272: 26627-26633 [Abstract] [Full Text]