This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Montenegro, E Articles by Martín, J F Search for Related Content PubMed PubMed Citation Articles by Montenegro, E Articles by Martín, J F

 Previous Article

J Bacteriol. 1992 November; 174(21): 7063-7067

research-article

Resolution of chromosomes III and VI of Aspergillus nidulans by pulsed-field gel electrophoresis shows that the penicillin biosynthetic pathway genes pcbAB, pcbC, and penDE are clustered on chromosome VI (3.0 megabases).

Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of León, Spain.

ABSTRACT

An improved electrophoretic molecular karyotype of Aspergillus nidulans ATCC 28901 has been obtained by contour-clamped electric field gel electrophoresis, which separates seven chromosomal bands and allows resolution of chromosomes III and VI. The three genes of the penicillin biosynthetic pathway, pcbAB, pcbC, and penDE, encoding alpha-aminoadipyl-cysteinyl-valine synthetase, isopenicillin N synthase, and isopenicillin N acyltransferase, respectively, are clustered together on a chromosome of 3.0 Mg, corresponding to linkage group VI, whereas the argB gene was located on a chromosome of 3.4 Mb, corresponding to linkage group III. Three other strains of A. nidulans contained a modified chromosome III of about 3.1 Mb that overlaps with chromosome VI, forming a doublet. Resolution of chromosomes III and VI in strain ATCC 28901 allowed unequivocal mapping of the penicillin gene cluster on chromosome VI of A. nidulans.

This article has been cited by other articles:

• Shimizu, K., Hicks, J. K., Huang, T.-P., Keller, N. P. (2003). Pka, Ras and RGS Protein Interactions Regulate Activity of AflR, a Zn(II)2Cys6 Transcription Factor in Aspergillus nidulans. Genetics 165: 1095-1104 [Abstract] [Full Text]  
• Brakhage, A. A. (1998). Molecular Regulation of beta -Lactam Biosynthesis in Filamentous Fungi. Microbiol. Mol. Biol. Rev. 62: 547-585 [Abstract] [Full Text]