This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yen, K M Articles by Karl, M R Search for Related Content PubMed PubMed Citation Articles by Yen, K M Articles by Karl, M R

research-article

Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.

Amgen Inc., Amgen Center, Thousand Oaks, California 91320-1789.

ABSTRACT

Five genes, tmoABCDE, encoding toluene-4-monooxygenase (T4MO) were previously mapped to a 3.6-kb region of a 10.2-kb SacI DNA fragment isolated from Pseudomonas mendocina KR1 (K.-M. Yen, M. R. Karl, L. M. Blatt, M. J. Simon, R. B. Winter, P. R. Fausset, H. S. Lu, A. A. Harcourt, and K. K. Chen, J. Bacteriol. 173:5315-5327, 1991). In this report, we describe the identification and characterization of a DNA region in the SacI fragment whose expression enhances the T4MO activity determined by the tmoABCDE gene cluster. This region was mapped immediately downstream of the putative transcription termination sequence previously located at the end of the tmoABCDE gene cluster (Yen et al., J. Bacteriol., 1991) and was found to stimulate T4MO activity two- to threefold when expressed in Escherichia coli or Pseudomonas putida. Determination of the nucleotide sequence of this region revealed an open reading frame (ORF) of 978 bp. Expression of the ORF resulted in the synthesis of an approximately 37-kDa polypeptide whose N-terminal amino acid sequence completely matched that of the product predicted from the ORF. The ORF thus defines a gene, which has now been designated tmoF. The TmoF protein shares amino acid sequence homology with the reductases of several mono- and dioxygenase systems. In addition, the reductase component of the naphthalene dioxygenase system, encoded by the nahAa gene of plasmid NAH7 from P. putida G7, could largely replace the TmoF protein in stimulating T4MO activity, and TmoF could partially replace the NahAa protein in forming active naphthalene dioxygenase. The overall properties of tmoF suggest that it is a member of the T4mo gene cluster and encodes the NADH:ferredoxin oxidoreductase of the T4MO system.

This article has been cited by other articles:

• Tao, Y., Fishman, A., Bentley, W. E., Wood, T. K. (2004). Oxidation of Benzene to Phenol, Catechol, and 1,2,3-Trihydroxybenzene by Toluene 4-Monooxygenase of Pseudomonas mendocina KR1 and Toluene 3-Monooxygenase of Ralstonia pickettii PKO1. Appl. Environ. Microbiol. 70: 3814-3820 [Abstract] [Full Text]  
• Chang, H.-K., Mohseni, P., Zylstra, G. J. (2003). Characterization and Regulation of the Genes for a Novel Anthranilate 1,2-Dioxygenase from Burkholderia cepacia DBO1. J. Bacteriol. 185: 5871-5881 [Abstract] [Full Text]  
• Baldwin, B. R., Nakatsu, C. H., Nies, L. (2003). Detection and Enumeration of Aromatic Oxygenase Genes by Multiplex and Real-Time PCR. Appl. Environ. Microbiol. 69: 3350-3358 [Abstract] [Full Text]  
• Ramos-Gonzalez, M.-I., Olson, M., Gatenby, A. A., Mosqueda, G., Manzanera, M., Campos, M. J., Vichez, S., Ramos, J. L. (2002). Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in Pseudomonas mendocina and the TodST System from Pseudomonas putida. J. Bacteriol. 184: 7062-7067 [Abstract] [Full Text]  
• Nam, J.-W., Nojiri, H., Noguchi, H., Uchimura, H., Yoshida, T., Habe, H., Yamane, H., Omori, T. (2002). Purification and Characterization of Carbazole 1,9a-Dioxygenase, a Three-Component Dioxygenase System of Pseudomonas resinovorans Strain CA10. Appl. Environ. Microbiol. 68: 5882-5890 [Abstract] [Full Text]  
• Sluis, M. K., Sayavedra-Soto, L. A., Arp, D. J. (2002). Molecular analysis of the soluble butane monooxygenase from 'Pseudomonas butanovora'. Microbiology 148: 3617-3629 [Abstract] [Full Text]  
• Kahng, H.-Y., Malinverni, J. C., Majko, M. M., Kukor, J. J. (2001). Genetic and Functional Analysis of the tbc Operons for Catabolism of Alkyl- and Chloroaromatic Compounds in Burkholderia sp. Strain JS150. Appl. Environ. Microbiol. 67: 4805-4816 [Abstract] [Full Text]  
• Scognamiglio, R., Notomista, E., Barbieri, P., Pucci, P., Piaz, F. D., Tramontano, A., Di Donato, A. (2001). Conformational analysis of putative regulatory subunit D of the toluene/o-xylene-monooxygenase complex from Pseudomonas stutzeri OX1. Protein Sci. 10: 482-490 [Abstract] [Full Text]  
• van Hylckama Vlieg, J. E. T., Leemhuis, H., Spelberg, J. H. L., Janssen, D. B. (2000). Characterization of the Gene Cluster Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45. J. Bacteriol. 182: 1956-1963 [Abstract] [Full Text]  
• Tsoi, T. V., Plotnikova, E. G., Cole, J. R., Guerin, W. F., Bagdasarian, M., Tiedje, J. M. (1999). Cloning, Expression, and Nucleotide Sequence of the Pseudomonas aeruginosa 142 ohb Genes Coding for Oxygenolytic ortho Dehalogenation of Halobenzoates. Appl. Environ. Microbiol. 65: 2151-2162 [Abstract] [Full Text]  
• Zhou, N.-Y., Jenkins, A., Chan Kwo Chion, C. K. N., Leak, D. J. (1999). The Alkene Monooxygenase from Xanthobacter Strain Py2 Is Closely Related to Aromatic Monooxygenases and Catalyzes Aromatic Monohydroxylation of Benzene, Toluene, and Phenol. Appl. Environ. Microbiol. 65: 1589-1595 [Abstract] [Full Text]  
• de Marco, P., Moradas-Ferreira, P., Higgins, T. P., McDonald, I., Kenna, E. M., Murrell, J. C. (1999). Molecular Analysis of a Novel Methanesulfonic Acid Monooxygenase from the Methylotroph Methylosulfonomonas methylovora. J. Bacteriol. 181: 2244-2251 [Abstract] [Full Text]  
• Bertoni, G., Martino, M., Galli, E., Barbieri, P. (1998). Analysis of the Gene Cluster Encoding Toluene/o-Xylene Monooxygenase from Pseudomonas stutzeri OX1. Appl. Environ. Microbiol. 64: 3626-3632 [Abstract] [Full Text]