This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Swenson, D L Articles by Clegg, S Search for Related Content PubMed PubMed Citation Articles by Swenson, D L Articles by Clegg, S

research-article

Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium.

Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242.

ABSTRACT

Regulation of the gene, fimA, encoding the major fimbrial subunit of S. typhimurium S6704 was examined by using a lambda fimA-lacZ lysogen. Transformation of the lambda fimA-lacZ lysogen with various derivatives of the recombinant plasmid that encodes type 1 fimbrial expression, pISF101, indicated that two regions of this plasmid alter beta-galactosidase production. One plasmid is a deletion resulting in the loss of a 28-kDa polypeptide downstream of fimA, while the other plasmid encodes a 24- and a 27-kDa polypeptide. Northern (RNA) blot analyses indicated that the steady-state fimA mRNA levels of these transformants were high. In addition, phenotypic expression of type 1 fimbriae by agar-grown cultures is observed only in those transformants bearing plasmids which show increased beta-galactosidase and fimA mRNA levels.

This article has been cited by other articles:

• Baxter, M. A., Jones, B. D. (2005). The fimYZ Genes Regulate Salmonella enterica Serovar Typhimurium Invasion in Addition to Type 1 Fimbrial Expression and Bacterial Motility. Infect. Immun. 73: 1377-1385 [Abstract] [Full Text]  
• Clegg, S., Hughes, K. T. (2002). FimZ Is a Molecular Link between Sticking and Swimming in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 184: 1209-1213 [Abstract] [Full Text]  
• Tinker, J. K., Hancox, L. S., Clegg, S. (2001). FimW Is a Negative Regulator Affecting Type 1 Fimbrial Expression in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 183: 435-442 [Abstract] [Full Text]  
• Nicholson, T. L., Baumler, A. J. (2001). Salmonella enterica Serotype Typhimurium Elicits Cross-Immunity against a Salmonella enterica Serotype Enteritidis Strain Expressing LP Fimbriae from the lac Promoter. Infect. Immun. 69: 204-212 [Abstract] [Full Text]  
• Tinker, J. K., Clegg, S. (2000). Characterization of FimY as a Coactivator of Type 1 Fimbrial Expression in Salmonella enterica Serovar Typhimurium. Infect. Immun. 68: 3305-3313 [Abstract] [Full Text]  
• Norris, T. L., Baumler, A. J. (1999). Phase variation of the lpf operon is a mechanism to evade cross-immunity between Salmonella serotypes. Proc. Natl. Acad. Sci. USA 96: 13393-13398 [Abstract] [Full Text]  
• Darwin, K. H., Miller, V. L. (1999). Molecular Basis of the Interaction of Salmonella with the Intestinal Mucosa. Clin. Microbiol. Rev. 12: 405-428 [Abstract] [Full Text]  
• Clouthier, S. C., Collinson, S. K., White, A. P., Banser, P. A., Kay, W. W. (1998). tRNAArg (fimU) and Expression of SEF14 and SEF21 in Salmonella enteritidis. J. Bacteriol. 180: 840-845 [Abstract] [Full Text]