This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dong, Q Articles by Toussaint, A Search for Related Content PubMed PubMed Citation Articles by Dong, Q Articles by Toussaint, A

 Previous Article  |  Next Article 

J Bacteriol. 1992 December; 174(24): 8133-8138

research-article

Cloning and sequencing of IS1086, an Alcaligenes eutrophus insertion element related to IS30 and IS4351.

Laboratoire de Génétique & Biotechnologie, SCK/CEN-Vlaamse Instelling voor Technologisch Onderzoek, Mol, Belgium.

ABSTRACT

A new insertion sequence (IS), designated IS1086, was isolated from Alcaligenes eutrophus CH34 by being trapped in plasmid pJV240, which contains the Bacillus subtilis sacB and sacR genes. The 1,106-bp IS1086 element contains partially matched (22 of 28 bp) terminal-inverted repeats and a long open reading frame. Hybridization data suggest the presence of one copy of IS1086 in the strain CH34 heavy-metal resistance plasmid pMOL28 and at least two copies in its chromosome. Analysis of the IS1086 nucleotide sequence revealed striking homology with two other IS elements, IS30 and IS4351, suggesting that they are three close members in a family of phylogenetically related insertion sequences. One open reading frame of the Spiroplasma citri phage SpV1-R8A2 B was also found to be related to this IS family but to a lesser extent. Comparison of the G+C contents of IS30 and IS1086 revealed that they conform to their respective hosts (46 versus 50% for IS30 and Escherichia coli and 64.5% for IS1086 and A. eutrophus). The pressure on the AT/GC ratio led to a very different codon usage in these two closely related IS elements. Results suggesting that IS1086 transposition might be activated by some forms of stress are discussed.

This article has been cited by other articles:

• Monchy, S., Benotmane, M. A., Janssen, P., Vallaeys, T., Taghavi, S., van der Lelie, D., Mergeay, M. (2007). Plasmids pMOL28 and pMOL30 of Cupriavidus metallidurans Are Specialized in the Maximal Viable Response to Heavy Metals. J. Bacteriol. 189: 7417-7425 [Abstract] [Full Text]  
• Razin, S., Yogev, D., Naot, Y. (1998). Molecular Biology and Pathogenicity of Mycoplasmas. Microbiol. Mol. Biol. Rev. 62: 1094-1156 [Abstract] [Full Text]  
• Rudant, E., Courvalin, P., Lambert, T. (1998). Characterization of IS18, an Element Capable of Activating the Silent aac(6')-Ij Gene of Acinetobacter sp. 13 Strain BM2716 by Transposition. Antimicrob. Agents Chemother. 42: 2759-2761 [Abstract] [Full Text]  
• Bertoni, G., Martino, M., Galli, E., Barbieri, P. (1998). Analysis of the Gene Cluster Encoding Toluene/o-Xylene Monooxygenase from Pseudomonas stutzeri OX1. Appl. Environ. Microbiol. 64: 3626-3632 [Abstract] [Full Text]