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J Bacteriol. 1992 February; 174(3): 1050-1052

research-article

Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6y to precorrin-8x is catalyzed by the cobL gene product.

Département Analyse, Centre de Recherche de Vitry-Alfortville, Vitry-sur-Seine, France.

ABSTRACT

A protein catalyzing methylation at C-5 and C-15 and decarboxylation of the acetic acid side chain at C-12 on precorrin-6y to yield precorrin-8x was purified to homogeneity from a recombinant strain of Pseudomonas denitrificans. It was sequenced at the N terminus and shown to be encoded by the cobL gene.

This article has been cited by other articles:

• Shearer, N., Hinsley, A. P., Van Spanning, R. J. M., Spiro, S. (1999). Anaerobic Growth of Paracoccus denitrificans Requires Cobalamin: Characterization of cobK and cobJ Genes. J. Bacteriol. 181: 6907-6913 [Abstract] [Full Text]  
• Battersby, A. (1994). How nature builds the pigments of life: the conquest of vitamin B12. Science 264: 1551-1557 [Abstract]