This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rauch, P J Articles by De Vos, W M Search for Related Content PubMed PubMed Citation Articles by Rauch, P J Articles by De Vos, W M

 Previous Article  |  Next Article 

J Bacteriol. 1992 February; 174(4): 1280-1287

research-article

Characterization of the novel nisin-sucrose conjugative transposon Tn5276 and its insertion in Lactococcus lactis.

Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.

ABSTRACT

A novel, chromosomally located conjugative transposon in Lactococcus lactis, Tn5276, was identified and characterized. It encodes the production of and immunity to nisin, a lanthionine-containing peptide with antimicrobial activity, and the capacity to utilize sucrose via a phosphotransferase system. Conjugal transfer of Tn5276 was demonstrated from L. lactis NIZO R5 to different L. lactis strains and a recombination-deficient mutant. The integration of Tn5276 into the plasmid-free strain MG1614 was analyzed by using probes based on the gene for the nisin precursor (nisA) and the gene for sucrose-6-phosphate hydrolase (sacA). The transposon inserted at various locations in the MG1614 chromosome and showed a preference for orientation-specific insertion into a single target site (designated site 1). By using restriction mapping in combination with field inversion gel electrophoresis and DNA cloning of various parts of the element including its left and right ends, a physical map of the 70-kb Tn5276 was constructed, and the nisA and sacA genes were located. The nucleotide sequences of Tn5276 junctions in donor strain NIZO R5 and in site 1 of an MG1614-derived transconjugant were determined and compared with that of site 1 in recipient strain MG1614. The results show that the A + T-rich ends of Tn5276 are flanked by a direct hexanucleotide repeat in both the donor and the transconjugant but that the element does not contain a clear inverted repeat.

This article has been cited by other articles:

• Ichiyanagi, K., Beauregard, A., Belfort, M. (2003). A bacterial group II intron favors retrotransposition into plasmid targets. Proc. Natl. Acad. Sci. USA 100: 15742-15747 [Abstract] [Full Text]  
• Beaber, J. W., Hochhut, B., Waldor, M. K. (2002). Genomic and Functional Analyses of SXT, an Integrating Antibiotic Resistance Gene Transfer Element Derived from Vibrio cholerae. J. Bacteriol. 184: 4259-4269 [Abstract] [Full Text]  
• Skaugen, M., Andersen, E. L., Christie, V. H., Nes, I. F. (2002). Identification, Characterization, and Expression of a Second, Bicistronic, Operon Involved in the Production of Lactocin S in Lactobacillus sakei L45. Appl. Environ. Microbiol. 68: 720-727 [Abstract] [Full Text]  
• Whittle, G., Hund, B. D., Shoemaker, N. B., Salyers, A. A. (2001). Characterization of the 13-Kilobase ermF Region of the Bacteroides Conjugative Transposon CTnDOT. Appl. Environ. Microbiol. 67: 3488-3495 [Abstract] [Full Text]  
• Le Bourgeois, P., Daveran-Mingot, M.-L., Ritzenthaler, P. (2000). Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms. J. Bacteriol. 182: 2481-2491 [Abstract] [Full Text]  
• Burrus, V., Roussel, Y., Decaris, B., Guédon, G. (2000). Characterization of a Novel Integrative Element, ICESt1, in the Lactic Acid Bacterium Streptococcus thermophilus. Appl. Environ. Microbiol. 66: 1749-1753 [Abstract] [Full Text]  
• Martínez, B., Fernández, M., Suárez, J. E., Rodríguez, A. (1999). Synthesis of lactococcin 972, a bacteriocin produced by Lactococcus lactis IPLA 972, depends on the expression of a plasmid-encoded bicistronic operon. Microbiology 145: 3155-3161 [Abstract] [Full Text]  
• Luesink, E. J., Marugg, J. D., Kuipers, O. P., de Vos, W. M. (1999). Characterization of the Divergent sacBK and sacAR Operons, Involved in Sucrose Utilization by Lactococcus lactis. J. Bacteriol. 181: 1924-1926 [Abstract] [Full Text]  
• Meijer, W., van de Bunt, B., Twigt, M., de Jonge, B., Smit, G., Hugenholtz, J. (1998). Lysis of Lactococcus lactis subsp. cremoris SK110 and Its Nisin-Immune Transconjugant in Relation to Flavor Development in Cheese. Appl. Environ. Microbiol. 64: 1950-1953 [Abstract] [Full Text]  
• Rice, L. B., Carias, L. L. (1998). Transfer of Tn5385, a Composite, Multiresistance Chromosomal Element from Enterococcus faecalis. J. Bacteriol. 180: 714-721 [Abstract] [Full Text]  
• Kuipers, O. P., Beerthuyzen, M. M., de Ruyter, P. G. G. A., Luesink, E. J., de Vos, W. M. (1995). Autoregulation of Nisin Biosynthesis in Lactococcus lactis by Signal Transduction. J. Biol. Chem. 270: 27299-27304 [Abstract] [Full Text]  
• Donkersloot, J. A., Thompson, J. (1995). Cloning, Expression, Sequence Analysis, and Site-directed Mutagenesis of the Tn5306-encoded N[IMAGE]-(Carboxyethyl)ornithine Synthase from Lactococcus lactis K1. J. Biol. Chem. 270: 12226-12234 [Abstract] [Full Text]