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J Bacteriol. 1992 February; 174(4): 1288-1292

research-article

Expression of bacterial mercuric ion reductase in Saccharomyces cerevisiae.

C Rensing, U Kües, U Stahl, D H Nies and B Friedrich

Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Germany.

ABSTRACT

The gene merA coding for bacterial mercuric ion reductase was cloned under the control of the yeast promoter for alcohol dehydrogenase I in the yeast-Escherichia coli shuttle plasmid pADH040-2 and transformed into Saccharomyces cerevisiae AH22. The resulting transformant harbored stable copies of the merA-containing hybrid plasmid, displayed a fivefold increase in the MIC of mercuric chloride, and synthesized mercuric ion reductase activity.

J Bacteriol. 1992 February; 174(4): 1288-1292






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