This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Henkin, T M Articles by Grundy, F J Search for Related Content PubMed PubMed Citation Articles by Henkin, T M Articles by Grundy, F J

 Previous Article  |  Next Article 

J Bacteriol. 1992 February; 174(4): 1299-1306

research-article

Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory sequence in multiple tRNA synthetase genes.

Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.

ABSTRACT

The Bacillus subtilis tyrS gene, which encodes tyrosyl-tRNA synthetase (TyrTS), was isolated, and its nucleotide sequence was determined. The cloned gene was shown to complement an Escherichia coli tyrS (Ts) mutant. The predicted amino acid sequence exhibited 70% identity to that of Bacillus stearothermophilus TyrTS and 55% identity to that of E. coli TyrTS, while identity to a second cryptic B. subtilis TyrTS gene, designated tyrZ, was only 27%. Primer extension analysis indicated that tyrS transcription initiated at a vegetative promoter sequence located 300 nucleotides upstream of the AUG start codon. The mRNA leader region was found to contain an inverted repeat sequence resembling a transcriptional terminator. Expression of a transcriptional tyrS-lacZ fusion was found to be induced by starvation for tyrosine in a tyrosine auxotroph (tyrA1). Transcription initiation was unaffected by tyrosine starvation. Deletion of the terminator region in a tyrS-lacZ fusion resulted in high-level constitutive expression. Immediately preceding the putative terminator was sequence element found to be conserved in the upstream region of a number of Bacillus tRNA synthetase genes as well as in the ilv-leu biosynthetic operon; mutation of this element in tyrS resulted in low-level uninducible expression. The conservation of this sequence element suggests that aminoacyl-tRNA synthetase genes and the ilv-leu operon may be regulated by a common mechanism in Bacillus spp.

This article has been cited by other articles:

• Vitreschak, A. G., Mironov, A. A., Lyubetsky, V. A., Gelfand, M. S. (2008). Comparative genomic analysis of T-box regulatory systems in bacteria. RNA 14: 717-735 [Abstract] [Full Text]  
• Porat, I., Vinogradov, M., Vyazmensky, M., Lu, C.-D., Chipman, D. M., Abdelal, A. T., Barak, Z.'e. (2004). Cloning and Characterization of Acetohydroxyacid Synthase from Bacillus stearothermophilus. J. Bacteriol. 186: 570-574 [Abstract] [Full Text]  
• Grundy, F. J., Winkler, W. C., Henkin, T. M. (2002). tRNA-mediated transcription antitermination in vitro: Codon-anticodon pairing independent of the ribosome. Proc. Natl. Acad. Sci. USA 99: 11121-11126 [Abstract] [Full Text]  
• Connil, N., Le Breton, Y., Dousset, X., Auffray, Y., Rince, A., Prevost, H. (2002). Identification of the Enterococcus faecalis Tyrosine Decarboxylase Operon Involved in Tyramine Production. Appl. Environ. Microbiol. 68: 3537-3544 [Abstract] [Full Text]  
• Grundy, F. J., Moir, T. R., Haldeman, M. T., Henkin, T. M. (2002). Sequence requirements for terminators and antiterminators in the T box transcription antitermination system: disparity between conservation and functional requirements. Nucleic Acids Res 30: 1646-1655 [Abstract] [Full Text]  
• Garault, P., Letort, C., Juillard, V., Monnet, V. (2000). Branched-Chain Amino Acid Biosynthesis Is Essential for Optimal Growth of Streptococcus thermophilus in Milk. Appl. Environ. Microbiol. 66: 5128-5133 [Abstract] [Full Text]  
• McGuire, A. M., Hughes, J. D., Church, G. M. (2000). Conservation of DNA Regulatory Motifs and Discovery of New Motifs in Microbial Genomes. Genome Res 10: 744-757 [Abstract] [Full Text]  
• Becker, H. D., Kern, D. (1998). Thermus thermophilus: A link in evolution of the tRNA-dependent amino acid amidation pathways. Proc. Natl. Acad. Sci. USA 95: 12832-12837 [Abstract] [Full Text]