This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Verbon, A Articles by Lathigra, R Search for Related Content PubMed PubMed Citation Articles by Verbon, A Articles by Lathigra, R

 Previous Article  |  Next Article 

J Bacteriol. 1992 February; 174(4): 1352-1359

research-article

The 14,000-molecular-weight antigen of Mycobacterium tuberculosis is related to the alpha-crystallin family of low-molecular-weight heat shock proteins.

N.H. Swellengrebel Institute of Tropical Hygeine, Royal Tropical Institute, Amsterdam, The Netherlands.

ABSTRACT

Eight monoclonal antibodies (MAbs) directed against the 14,000-molecular-weight (14K) antigen of Mycobacterium tuberculosis reacted specifically with mycobacteria of the M. tuberculosis complex. The nucleotide sequence of the gene encoding the 14K antigen was determined by using recombinant DNA clones isolated from lambda gt11 and cosmid libraries of the M. tuberculosis genome. The DNA sequence of the 14K protein gene coded for a polypeptide of 144 amino acids with a calculated molecular mass of 16,277 Da. The 14K antigen has a marked homology with proteins belonging to the alpha-crystallin family of low-molecular-weight heat shock proteins, which includes the 18K antigen of M. leprae. The eight MAbs recognized at least four distinct epitopes localized within the following three regions of the 14K protein: amino acids 10 to 92 (MAbs F67-8 and F67-16), amino acids 41 to 92 (F159-1 and F159-11), and amino acids 41 to 144 (F23-41, F24-2, F23-49, and TB68).

This article has been cited by other articles:

• Pang, X., Howard, S. T. (2007). Regulation of the {alpha}-Crystallin Gene acr2 by the MprAB Two-Component System of Mycobacterium tuberculosis. J. Bacteriol. 189: 6213-6221 [Abstract] [Full Text]  
• Stewart, J. N., Rivera, H. N., Karls, R., Quinn, F. D., Roman, J., Rivera-Marrero, C. A. (2006). Increased pathology in lungs of mice after infection with an {alpha}-crystallin mutant of Mycobacterium tuberculosis: changes in cathepsin proteases and certain cytokines. Microbiology 152: 233-244 [Abstract] [Full Text]  
• Saha, A., Sharma, A., Dhar, A., Bhattacharyya, B., Roy, S., Das Gupta, S. K. (2005). Antagonists of Hsp16.3, a Low-Molecular-Weight Mycobacterial Chaperone and Virulence Factor, Derived from Phage-Displayed Peptide Libraries. Appl. Environ. Microbiol. 71: 7334-7344 [Abstract] [Full Text]  
• Kennaway, C. K., Benesch, J. L. P., Gohlke, U., Wang, L., Robinson, C. V., Orlova, E. V., Saibi, H. R., Keep, N. H. (2005). Dodecameric Structure of the Small Heat Shock Protein Acr1 from Mycobacterium tuberculosis. J. Biol. Chem. 280: 33419-33425 [Abstract] [Full Text]  
• Wilkinson, K. A., Stewart, G. R., Newton, S. M., Vordermeier, H. M., Wain, J. R., Murphy, H. N., Horner, K., Young, D. B., Wilkinson, R. J. (2005). Infection Biology of a Novel {alpha}-Crystallin of Mycobacterium tuberculosis: Acr2. J. Immunol. 174: 4237-4243 [Abstract] [Full Text]  
• Preneta, R., Papavinasasundaram, K. G., Cozzone, A. J., Duclos, B. (2004). Autophosphorylation of the 16 kDa and 70 kDa antigens (Hsp 16{middle dot}3 and Hsp 70) of Mycobacterium tuberculosis. Microbiology 150: 2135-2141 [Abstract] [Full Text]  
• Imaz, M. S., Comini, M. A., Zerbini, E., Sequeira, M. D., Latini, O., Claus, J. D., Singh, M. (2004). Evaluation of Commercial Enzyme-Linked Immunosorbent Assay Kits for Detection of Tuberculosis in Argentinean Population. J. Clin. Microbiol. 42: 884-887 [Abstract] [Full Text]  
• Vekemans, J., Ota, M. O. C., Sillah, J., Fielding, K., Alderson, M. R., Skeiky, Y. A. W., Dalemans, W., McAdam, K. P. W. J., Lienhardt, C., Marchant, A. (2004). Immune Responses to Mycobacterial Antigens in the Gambian Population: Implications for Vaccines and Immunodiagnostic Test Design. Infect. Immun. 72: 381-388 [Abstract] [Full Text]  
• Yeremeev, V. V., Kondratieva, T. K., Rubakova, E. I., Petrovskaya, S. N., Kazarian, K. A., Telkov, M. V., Biketov, S. F., Kaprelyants, A. S., Apt, A. S. (2003). Proteins of the Rpf Family: Immune Cell Reactivity and Vaccination Efficacy against Tuberculosis in Mice. Infect. Immun. 71: 4789-4794 [Abstract] [Full Text]  
• Raja, A., Uma Devi, K. R., Ramalingam, B., Brennan, P. J. (2002). Immunoglobulin G, A, and M Responses in Serum and Circulating Immune Complexes Elicited by the 16-Kilodalton Antigen of Mycobacterium tuberculosis. CVI 9: 308-312 [Abstract] [Full Text]  
• Narberhaus, F. (2002). {alpha}-Crystallin-Type Heat Shock Proteins: Socializing Minichaperones in the Context of a Multichaperone Network. Microbiol. Mol. Biol. Rev. 66: 64-93 [Abstract] [Full Text]  
• Lodes, M. J., Dillon, D. C., Mohamath, R., Day, C. H., Benson, D. R., Reynolds, L. D., McNeill, P., Sampaio, D. P., Skeiky, Y. A. W., Badaro, R., Persing, D. H., Reed, S. G., Houghton, R. L. (2001). Serological Expression Cloning and Immunological Evaluation of MTB48, a Novel Mycobacterium tuberculosis Antigen. J. Clin. Microbiol. 39: 2485-2493 [Abstract] [Full Text]  
• Sherman, D. R., Voskuil, M., Schnappinger, D., Liao, R., Harrell, M. I., Schoolnik, G. K. (2001). Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin. Proc. Natl. Acad. Sci. USA 98: 7534-7539 [Abstract] [Full Text]  
• Rhodes, S. G., Gavier-Widen, D., Buddle, B. M., Whelan, A. O., Singh, M., Hewinson, R. G., Vordermeier, H. M. (2000). Antigen Specificity in Experimental Bovine Tuberculosis. Infect. Immun. 68: 2573-2578 [Abstract] [Full Text]  
• Musser, J. M., Amin, A., Ramaswamy, S. (2000). Negligible Genetic Diversity of Mycobacterium tuberculosis Host Immune System Protein Targets: Evidence of Limited Selective Pressure. Genetics 155: 7-16 [Abstract] [Full Text]  
• Hu, Y., Coates, A. R. M. (1999). Transcription of the Stationary-Phase-Associated hspX Gene of Mycobacterium tuberculosis Is Inversely Related to Synthesis of the 16-Kilodalton Protein. J. Bacteriol. 181: 1380-1387 [Abstract] [Full Text]  
• Wong, D. K., Lee, B.-Y., Horwitz, M. A., Gibson, B. W. (1999). Identification of Fur, Aconitase, and Other Proteins Expressed by Mycobacterium tuberculosis under Conditions of Low and High Concentrations of Iron by Combined Two-Dimensional Gel Electrophoresis and Mass Spectrometry. Infect. Immun. 67: 327-336 [Abstract] [Full Text]  
• Garbe, T. R., Hibler, N. S., Deretic, V. (1999). Response to Reactive Nitrogen Intermediates in Mycobacterium tuberculosis: Induction of the 16-Kilodalton alpha -Crystallin Homolog by Exposure to Nitric Oxide Donors. Infect. Immun. 67: 460-465 [Abstract] [Full Text]  
• Lyashchenko, K. P., Pollock, J. M., Colangeli, R., Gennaro, M. L. (1998). Diversity of Antigen Recognition by Serum Antibodies in Experimental Bovine Tuberculosis. Infect. Immun. 66: 5344-5349 [Abstract] [Full Text]  
• Yuan, Y., Crane, D. D., Simpson, R. M., Zhu, Y., Hickey, M. J., Sherman, D. R., Barry, C. E. III (1998). The 16-kDa alpha -crystallin (Acr) protein of Mycobacterium tuberculosis is required for growth in macrophages. Proc. Natl. Acad. Sci. USA 95: 9578-9583 [Abstract] [Full Text]  
• Lyashchenko, K., Colangeli, R., Houde, M., Al Jahdali, H., Menzies, D., Gennaro, M. L. (1998). Heterogeneous Antibody Responses in Tuberculosis. Infect. Immun. 66: 3936-3940 [Abstract] [Full Text]  
• Roy, S. K., Nakamoto, H. (1998). Cloning, Characterization, and Transcriptional Analysis of a Gene Encoding an alpha -Crystallin-Related, Small Heat Shock Protein from the Thermophilic Cyanobacterium Synechococcus vulcanus. J. Bacteriol. 180: 3997-4001 [Abstract] [Full Text]  
• Cunningham, A. F., Spreadbury, C. L. (1998). Mycobacterial Stationary Phase Induced by Low Oxygen Tension: Cell Wall Thickening and Localization of the 16-Kilodalton alpha -Crystallin Homolog. J. Bacteriol. 180: 801-808 [Abstract] [Full Text]  
• Chang, Z., Primm, T. P., Jakana, J., Lee, I. H., Serysheva, I., Chiu, W., Gilbert, H. F., Quiocho, F. A. (1996). Mycobacterium tuberculosis 16-kDa Antigen (Hsp16.3) Functions as an Oligomeric Structure in Vitro to Suppress Thermal Aggregation. J. Biol. Chem. 271: 7218-7223 [Abstract] [Full Text]