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J Bacteriol. 1992 March; 174(5): 1682-1685

research-article

Quantitation of Dam methyltransferase in Escherichia coli.

E Boye, M G Marinus and A Løbner-Olesen

Department of Biophysics, Institute for Cancer Research, Oslo, Norway.

ABSTRACT

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.


J Bacteriol. 1992 March; 174(5): 1682-1685




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Copyright © 1992 by the American Society for Microbiology. All rights reserved.