This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Young, S A Articles by Bender, C L Search for Related Content PubMed PubMed Citation Articles by Young, S A Articles by Bender, C L

research-article

Physical and functional characterization of the gene cluster encoding the polyketide phytotoxin coronatine in Pseudomonas syringae pv. glycinea.

Department of Plant Pathology, Oklahoma State University, Stillwater 74078-9947.

ABSTRACT

Pseudomonas syringae pv. glycinea PG4180 produces the polyketide phytotoxin coronatine. The coronatine synthesis genes in PG4180 were previously shown to reside on a 90-kb plasmid designated p4180A. In the present study, clones containing a 34-kb region of p4180A were saturated with Tn5, and 71 unique mutations were recombined into p4180A by marker exchange. The effect of each mutation on coronatine synthesis was determined by analyzing the organic acids produced by the mutants by reverse-phase high-performance liquid chromatography. The organic acids of selected mutants were derivatized to their methyl esters and analyzed by gas chromatography and gas chromatography-mass spectrometry. Mutations in a 20.5-kb region of p4180A completely blocked the synthesis of coronafacic acid and coronatine. Mutations within a 4.4-kb region of p4180A prevented the formation of coronatine but allowed for production of coronafacic acid, coronafacoylvaline, coronafacoylisoleucine, and coronafacoylalloisoleucine. The phenotypes of selected mutants were further confirmed in feeding experiments in which coronafacic acid or coronamic acid was added to the culture media. The results of this study allow us to speculate on the likely sequence of steps in the later stages of coronatine biosynthesis.

This article has been cited by other articles:

• Seidle, H., Rangaswamy, V., Couch, R., Bender, C. L., Parry, R. J. (2004). Characterization of Cfa1, a Monofunctional Acyl Carrier Protein Involved in the Biosynthesis of the Phytotoxin Coronatine. J. Bacteriol. 186: 2499-2503 [Abstract] [Full Text]  
• Couch, R., O'Connor, S. E., Seidle, H., Walsh, C. T., Parry, R. (2004). Characterization of CmaA, an Adenylation-Thiolation Didomain Enzyme Involved in the Biosynthesis of Coronatine. J. Bacteriol. 186: 35-42 [Abstract] [Full Text]  
• Bender, C. L., Alarcon-Chaidez, F., Gross, D. C. (1999). Pseudomonas syringae Phytotoxins: Mode of Action, Regulation, and Biosynthesis by Peptide and Polyketide Synthetases. Microbiol. Mol. Biol. Rev. 63: 266-292 [Abstract] [Full Text]  
• Rohde, B. H., Schmid, R., Ullrich, M. S. (1999). Thermoregulated Expression and Characterization of an NAD(P)H-Dependent 2-Cyclohexen-1-one Reductase in the Plant Pathogenic Bacterium Pseudomonas syringae pv. glycinea. J. Bacteriol. 181: 814-822 [Abstract] [Full Text]  
• Rangaswamy, V., Jiralerspong, S., Parry, R., Bender, C. L. (1998). Biosynthesis of the Pseudomonas polyketide coronafacic acid requires monofunctional and multifunctional polyketide synthase proteins. Proc. Natl. Acad. Sci. USA 95: 15469-15474 [Abstract] [Full Text]  
• Peñaloza-Vázquez, A., Bender, C. L. (1998). Characterization of CorR, a Transcriptional Activator Which Is Required for Biosynthesis of the Phytotoxin Coronatine. J. Bacteriol. 180: 6252-6259 [Abstract] [Full Text]  
• Rangaswamy, V., Mitchell, R., Ullrich, M., Bender, C. (1998). Analysis of Genes Involved in Biosynthesis of Coronafacic Acid, the Polyketide Component of the Phytotoxin Coronatine. J. Bacteriol. 180: 3330-3338 [Abstract] [Full Text]