This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lam, J S Articles by MacDonald, L A Search for Related Content PubMed PubMed Citation Articles by Lam, J S Articles by MacDonald, L A

 Previous Article  |  Next Article 

J Bacteriol. 1992 April; 174(7): 2178-2184

research-article

Monoclonal antibodies as probes to examine serotype-specific and cross-reactive epitopes of lipopolysaccharides from serotypes O2, O5, and O16 of Pseudomonas aeruginosa.

Department of Microbiology, University of Guelph, Ontario, Canada.

ABSTRACT

Serotypes O2, O5, and O16 of Pseudomonas aeruginosa are chemically related, and the O antigens of their lipopolysaccharides share a similar trisaccharide repeat backbone structure. Serotype-specific monoclonal antibodies (MAbs) MF71-3, MF15-4, and MF47-4 against the O2, O5, and O16 serotypes, respectively, were isolated. MAb 18-19, which is cross-reactive with all strains of this chemically related serogroup, was also produced. When column chromatography or sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated lipopolysaccharide (LPS) samples from each of the serotypes were probed with the MAbs in Western immunoblots, each of the serotype-specific MAbs interacted only with high-molecular-weight bands of the homologous LPS, with a minimum O-antigen chain length of at least 6 to 10 repeats. In contrast, cross-reactive MAb 18-19 was shown to interact in Western immunoblots with the entire LPS banding pattern except the fastest-running band, which lacks O antigen. Chemical modification of P. aeruginosa LPS by alkali treatment and carboxyl reduction abolished reactions between LPS and MAb 18-19, while reactions of modified LPS with serotype-specific MAbs were not affected. Therefore, cross-reactive MAb 18-19 likely recognizes the chemical backbone structure of the O repeat that is common to all three serotypes of the O2-O5-O16 group, while the O-specific MAbs appeared to recognize LPS epitopes that could be presented when 6 to 10 or more O-antigen repeat units are present on the LPS molecule. Thus, the O-specific LPS epitopes likely involve unique chemical structures, glycosidic linkages, and some order of folding of the O side chains.

This article has been cited by other articles:

• Kaluzny, K., Abeyrathne, P. D., Lam, J. S. (2007). Coexistence of Two Distinct Versions of O-Antigen Polymerase, Wzy-Alpha and Wzy-Beta, in Pseudomonas aeruginosa Serogroup O2 and Their Contributions to Cell Surface Diversity. J. Bacteriol. 189: 4141-4152 [Abstract] [Full Text]  
• Burrows, L. L., Urbanic, R. V., Lam, J. S. (2000). Functional Conservation of the Polysaccharide Biosynthetic Protein WbpM and Its Homologues in Pseudomonas aeruginosa and Other Medically Significant Bacteria. Infect. Immun. 68: 931-936 [Abstract] [Full Text]  
• Rocchetta, H. L., Burrows, L. L., Lam, J. S. (1999). Genetics of O-Antigen Biosynthesis in Pseudomonas aeruginosa. Microbiol. Mol. Biol. Rev. 63: 523-553 [Abstract] [Full Text]  
• Burrows, L. L., Lam, J. S. (1999). Effect of wzx (rfbX) Mutations on A-Band and B-Band Lipopolysaccharide Biosynthesis in Pseudomonas aeruginosa O5. J. Bacteriol. 181: 973-980 [Abstract] [Full Text]  
• Emara, M.G., Malburg, S.R.C., Lam, J.S. (1995). Expression of an anti-Pseudomonas aeruginosa lipopolysaccharide core recombinant antibody in Escherichia coli. Innate Immunity 2: 53-61 [Abstract]