This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakamura, K Articles by Yamane, K Search for Related Content PubMed PubMed Citation Articles by Nakamura, K Articles by Yamane, K

research-article

Small cytoplasmic RNA of Bacillus subtilis: functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA.

Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.

ABSTRACT

Small cytoplasmic RNA (scRNA; 271 nucleotides) is an abundant and stable RNA of the gram-positive bacterium Bacillus subtilis. To investigate the function of scRNA in B. subtilis cells, we developed a strain that is dependent on isopropyl-beta-D-thiogalactopyranoside for scRNA synthesis by fusing the chromosomal scr locus with the spac-1 promoter by homologous recombination. Depletion of the inducer leads to a loss of scRNA synthesis, defects in protein synthesis and production of alpha-amylase and beta-lactamase, and eventual cell death. The loss of the scRNA gene in B. subtilis can be complemented by the introduction of human signal recognition particle 7S RNA, which is considered to be involved in protein transport, or Escherichia coli 4.5S RNA. These results provide further evidence for a functional relationship between B. subtilis scRNA, human signal recognition particle 7S RNA, and E. coli 4.5S RNA.

This article has been cited by other articles:

• Silvaggi, J. M., Perkins, J. B., Losick, R. (2006). Genes for Small, Noncoding RNAs under Sporulation Control in Bacillus subtilis. J. Bacteriol. 188: 532-541 [Abstract] [Full Text]  
• Zanen, G., Antelmann, H., Westers, H., Hecker, M., van Dijl, J. M., Quax, W. J. (2004). FlhF, the Third Signal Recognition Particle-GTPase of Bacillus subtilis, Is Dispensable for Protein Secretion. J. Bacteriol. 186: 5956-5960 [Abstract] [Full Text]  
• SAGAR, M. B., LUCAST, L., DOUDNA, J. A. (2004). Conserved but nonessential interaction of SRP RNA with translation factor EF-G. RNA 10: 772-778 [Abstract] [Full Text]  
• Condon, C. (2003). RNA Processing and Degradation in Bacillus subtilis. Microbiol. Mol. Biol. Rev. 67: 157-174 [Abstract] [Full Text]  
• Du, Y., Arvidson, C. G. (2003). Identification of ZipA, a Signal Recognition Particle-Dependent Protein from Neisseria gonorrhoeae. J. Bacteriol. 185: 2122-2130 [Abstract] [Full Text]  
• Kakeshita, H., Oguro, A., Amikura, R., Nakamura, K., Yamane, K. (2000). Expression of the ftsY gene, encoding a homologue of the {alpha} subunit of mammalian signal recognition particle receptor, is controlled by different promoters in vegetative and sporulating cells of Bacillus subtilis. Microbiology 146: 2595-2603 [Abstract] [Full Text]  
• Tjalsma, H., Bolhuis, A., Jongbloed, J. D. H., Bron, S., van Dijl, J. M. (2000). Signal Peptide-Dependent Protein Transport in Bacillus subtilis: a Genome-Based Survey of the Secretome. Microbiol. Mol. Biol. Rev. 64: 515-547 [Abstract] [Full Text]  
• Nakamura, K., Yahagi, S.-i., Yamazaki, T., Yamane, K. (1999). Bacillus subtilis Histone-like Protein, HBsu, Is an Integral Component of a SRP-like Particle That Can Bind the Alu Domain of Small Cytoplasmic RNA. J. Biol. Chem. 274: 13569-13576 [Abstract] [Full Text]  
• Kurita, K., Honda, K., Suzuma, S., Takamatsu, H., Nakamura, K., Yamane, K. (1996). Identification of a Region of Bacillus subtilis Ffh, a Homologue of Mammalian SRP54 Protein, That Is Essential for Binding to Small Cytoplasmic RNA. J. Biol. Chem. 271: 13140-13146 [Abstract] [Full Text]  
• Shibata, T., Fujii, Y., Nakamura, Y., Nakamura, K., Yamane, K. (1996). Identification of Protein Synthesis Elongation Factor G as a 4.5 S RNA-binding Protein in Escherichia coli. J. Biol. Chem. 271: 13162-13168 [Abstract] [Full Text]  
• Nakamura, K., Miyamoto, H., Suzuma, S., Sakamoto, T., Kawai, G., Yamane, K. (2001). Minimal Functional Structure of Escherichia coli 4.5 S RNA Required for Binding to Elongation Factor G. J. Biol. Chem. 276: 22844-22849 [Abstract] [Full Text]