This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wu, Z L Articles by Nester, E W Search for Related Content PubMed PubMed Citation Articles by Wu, Z L Articles by Nester, E W

research-article

The ntrA gene of Agrobacterium tumefaciens: identification, cloning, and phenotype of a site-directed mutant.

Department of Microbiology, University of Washington, Seattle 98195.

ABSTRACT

A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization and C4-dicarboxylate transport but not for vir gene expression or tumorigenesis.

This article has been cited by other articles:

• Jyot, J., Dasgupta, N., Ramphal, R. (2002). FleQ, the Major Flagellar Gene Regulator in Pseudomonas aeruginosa, Binds to Enhancer Sites Located Either Upstream or Atypically Downstream of the RpoN Binding Site. J. Bacteriol. 184: 5251-5260 [Abstract] [Full Text]  
• Hendrickson, E. L., Plotnikova, J., Mahajan-Miklos, S., Rahme, L. G., Ausubel, F. M. (2001). Differential Roles of the Pseudomonas aeruginosa PA14 rpoN Gene in Pathogenicity in Plants, Nematodes, Insects, and Mice. J. Bacteriol. 183: 7126-7134 [Abstract] [Full Text]  
• Hendrickson, E. L., Guevera, P., Peñaloza-Vàzquez, A., Shao, J., Bender, C., Ausubel, F. M. (2000). Virulence of the Phytopathogen Pseudomonas syringae pv. Maculicola Is rpoN Dependent. J. Bacteriol. 182: 3498-3507 [Abstract] [Full Text]  
• Hendrickson, E. L., Guevera, P., Ausubel, F. M. (2000). The Alternative Sigma Factor RpoN Is Required for hrp Activity in Pseudomonas syringae pv. Maculicola and Acts at the Level of hrpL Transcription. J. Bacteriol. 182: 3508-3516 [Abstract] [Full Text]  
• Michiels, J., Van Soom, T., D'hooghe, I., Dombrecht, B., Benhassine, T., de Wilde, P., Vanderleyden, J. (1998). The Rhizobium etli rpoN Locus: DNA Sequence Analysis and Phenotypical Characterization of rpoN, ptsN, and ptsA Mutants. J. Bacteriol. 180: 1729-1740 [Abstract] [Full Text]