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J Bacteriol. 1993 June; 175(11): 3672-3678

research-article

Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium.

Laboratoire de Biotechnologie des Champignons Filamenteux, Faculté des Sciences de Luminy, Parc Scientifique et Technologique, Marseille, France.

ABSTRACT

Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.

This article has been cited by other articles:

• Janse, B. J. H., Gaskell, J., Akhtar, M., Cullen, D. (1998). Expression of Phanerochaete chrysosporium Genes Encoding Lignin Peroxidases, Manganese Peroxidases, and Glyoxal Oxidase in Wood. Appl. Environ. Microbiol. 64: 3536-3538 [Abstract] [Full Text]