This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chow, C M Articles by RajBhandary, U L Search for Related Content PubMed PubMed Citation Articles by Chow, C M Articles by RajBhandary, U L

 Previous Article  |  Next Article 

J Bacteriol. 1993 June; 175(12): 3703-3709

research-article

Developmental regulation of the gene for formate dehydrogenase in Neurospora crassa.

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

ABSTRACT

We have isolated and characterized a gene, fdh, from Neurospora crassa which is developmentally regulated and which produces formate dehydrogenase activity when expressed in Escherichia coli. The gene is closely linked (less than 0.6 kb apart) to the leu-5 gene encoding mitochondrial leucyl-tRNA synthetase; the two genes are transcribed convergently from opposite strands. The expression patterns of these genes differ: fdh mRNA is found only during conidiation and early germination and is not detectable during mycelial growth, while leu-5 mRNA appears during germination and mycelial growth. The structure of the fdh gene was determined from the sequence of cDNA and genomic DNA clones and from mRNA mapping studies. The gene encodes a 375-amino-acid-long protein with sequence similarity to NAD-dependent dehydrogenases of the E. coli 3-phosphoglycerate dehydrogenase (serA gene product) subfamily. In particular, there is striking sequence similarity (52% identity) to formate dehydrogenase from Pseudomonas sp. strain 101. All of the residues thought to interact with NAD in the crystal structure of the Pseudomonas enzyme are conserved in the N. crassa enzyme. We have further shown that expression of the N. crassa gene in E. coli leads to the production of formate dehydrogenase activity, indicating that the N. crassa gene specifies a functional polypeptide.

This article has been cited by other articles:

• Harris, D. M., van der Krogt, Z. A., van Gulik, W. M., van Dijken, J. P., Pronk, J. T. (2007). Formate as an Auxiliary Substrate for Glucose-Limited Cultivation of Penicillium chrysogenum: Impact on Penicillin G Production and Biomass Yield. Appl. Environ. Microbiol. 73: 5020-5025 [Abstract] [Full Text]  
• Shinoda, T., Arai, K., Shigematsu-Iida, M., Ishikura, Y., Tanaka, S., Yamada, T., Kimber, M. S., Pai, E. F., Fushinobu, S., Taguchi, H. (2005). Distinct Conformation-mediated Functions of an Active Site Loop in the Catalytic Reactions of NAD-dependent D-Lactate Dehydrogenase and Formate Dehydrogenase. J. Biol. Chem. 280: 17068-17075 [Abstract] [Full Text]  
• Hwang, L., Hocking-Murray, D., Bahrami, A. K., Andersson, M., Rine, J., Sil, A. (2003). Identifying Phase-specific Genes in the Fungal Pathogen Histoplasma capsulatum Using a Genomic Shotgun Microarray. Mol. Biol. Cell 14: 2314-2326 [Abstract] [Full Text]  
• Suzuki, K., Itai, R., Suzuki, K., Nakanishi, H., Nishizawa, N.-K., Yoshimura, E., Mori, S. (1998). Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is Induced by Iron Deficiency in Barley Roots. Plant Physiol. 116: 725-732 [Abstract] [Full Text]