This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heym, B Articles by Cole, S T Search for Related Content PubMed PubMed Citation Articles by Heym, B Articles by Cole, S T

research-article

Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis.

Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.

ABSTRACT

The isoniazid susceptibility of Mycobacterium tuberculosis is mediated by the product of the katG gene which encodes the heme-containing enzyme catalase-peroxidase. In this study, the chromosomal location of katG has been established and its nucleotide sequence has been determined so that the primary structure of catalase-peroxidase could be predicted. The M. tuberculosis enzyme is an 80,000-dalton protein containing several motifs characteristic of peroxidases and shows strong similarity to other bacterial catalase-peroxidases. Expression of the katG gene in M. tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid.

This article has been cited by other articles:

• Drake, W. P., Dhason, M. S., Nadaf, M., Shepherd, B. E., Vadivelu, S., Hajizadeh, R., Newman, L. S., Kalams, S. A. (2007). Cellular Recognition of Mycobacterium tuberculosis ESAT-6 and KatG Peptides in Systemic Sarcoidosis. Infect. Immun. 75: 527-530 [Abstract] [Full Text]  
• Brossier, F., Veziris, N., Truffot-Pernot, C., Jarlier, V., Sougakoff, W. (2006). Performance of the Genotype MTBDR Line Probe Assay for Detection of Resistance to Rifampin and Isoniazid in Strains of Mycobacterium tuberculosis with Low- and High-Level Resistance.. J. Clin. Microbiol. 44: 3659-3664 [Abstract] [Full Text]  
• Hanoulle, X., Wieruszeski, J.-M., Rousselot-Pailley, P., Landrieu, I., Locht, C., Lippens, G., Baulard, A. R. (2006). Selective intracellular accumulation of the major metabolite issued from the activation of the prodrug ethionamide in mycobacteria. J Antimicrob Chemother 58: 768-772 [Abstract] [Full Text]  
• Sandy, J., Holton, S., Fullam, E., Sim, E., Noble, M. (2005). Binding of the anti-tubercular drug isoniazid to the arylamine N-acetyltransferase protein from Mycobacterium smegmatis. Protein Sci. 14: 775-782 [Abstract] [Full Text]  
• Huard, R. C., Chitale, S., Leung, M., Lazzarini, L. C. O., Zhu, H., Shashkina, E., Laal, S., Conde, M. B., Kritski, A. L., Belisle, J. T., Kreiswirth, B. N., Lapa e Silva, J. R., Ho, J. L. (2003). The Mycobacterium tuberculosis Complex-Restricted Gene cfp32 Encodes an Expressed Protein That Is Detectable in Tuberculosis Patients and Is Positively Correlated with Pulmonary Interleukin-10. Infect. Immun. 71: 6871-6883 [Abstract] [Full Text]  
• Yu, S., Girotto, S., Zhao, X., Magliozzo, R. S. (2003). Rapid Formation of Compound II and a Tyrosyl Radical in the Y229F Mutant of Mycobacterium tuberculosis Catalase-peroxidase Disrupts Catalase but Not Peroxidase Function. J. Biol. Chem. 278: 44121-44127 [Abstract] [Full Text]  
• Bakonyte, D., Baranauskaite, A., Cicenaite, J., Sosnovskaja, A., Stakenas, P. (2003). Molecular Characterization of Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates in Lithuania. Antimicrob. Agents Chemother. 47: 2009-2011 [Abstract] [Full Text]  
• Chouchane, S., Girotto, S., Kapetanaki, S., Schelvis, J. P. M., Yu, S., Magliozzo, R. S. (2003). Analysis of Heme Structural Heterogeneity in Mycobacterium tuberculosis Catalase-Peroxidase (KatG). J. Biol. Chem. 278: 8154-8162 [Abstract] [Full Text]  
• Wei, C.-J., Lei, B., Musser, J. M., Tu, S.-C. (2003). Isoniazid Activation Defects in Recombinant Mycobacterium tuberculosis Catalase-Peroxidase (KatG) Mutants Evident in InhA Inhibitor Production. Antimicrob. Agents Chemother. 47: 670-675 [Abstract] [Full Text]  
• DeVito, J. A., Morris, S. (2003). Exploring the Structure and Function of the Mycobacterial KatG Protein Using trans-Dominant Mutants. Antimicrob. Agents Chemother. 47: 188-195 [Abstract] [Full Text]  
• Chouchane, S., Girotto, S., Yu, S., Magliozzo, R. S. (2002). Identification and Characterization of Tyrosyl Radical Formation in Mycobacterium tuberculosis Catalase-Peroxidase (KatG). J. Biol. Chem. 277: 42633-42638 [Abstract] [Full Text]  
• Master, S. S., Springer, B., Sander, P., Boettger, E. C., Deretic, V., Timmins, G. S. (2002). Oxidative stress response genes in Mycobacterium tuberculosis: role of ahpC in resistance to peroxynitrite and stage-specific survival in macrophages. Microbiology 148: 3139-3144 [Abstract] [Full Text]  
• Bulatovic, V. M., Wengenack, N. L., Uhl, J. R., Hall, L., Roberts, G. D., Cockerill, F. R. III, Rusnak, F. (2002). Oxidative Stress Increases Susceptibility of Mycobacterium tuberculosis to Isoniazid. Antimicrob. Agents Chemother. 46: 2765-2771 [Abstract] [Full Text]  
• Milano, A., Forti, F., Sala, C., Riccardi, G., Ghisotti, D. (2001). Transcriptional Regulation of furA and katG upon Oxidative Stress in Mycobacterium smegmatis. J. Bacteriol. 183: 6801-6806 [Abstract] [Full Text]  
• Master, S., Zahrt, T. C., Song, J., Deretic, V. (2001). Mapping of Mycobacterium tuberculosis katG Promoters and Their Differential Expression in Infected Macrophages. J. Bacteriol. 183: 4033-4039 [Abstract] [Full Text]  
• Sim, E., Payton, M., Noble, M., Minchin, R. (2000). An update on genetic, structural and functional studies of arylamine N-acetyltransferases in eucaryotes and procaryotes. Hum Mol Genet 9: 2435-2441 [Abstract] [Full Text]  
• Hahn, J.-S., Oh, S.-Y., Roe, J.-H. (2000). Regulation of the furA and catC Operon, Encoding a Ferric Uptake Regulator Homologue and Catalase-Peroxidase, Respectively, in Streptomyces coelicolor A3(2). J. Bacteriol. 182: 3767-3774 [Abstract] [Full Text]  
• Couture, M., Yeh, S.-R., Wittenberg, B. A., Wittenberg, J. B., Ouellet, Y., Rousseau, D. L., Guertin, M. (1999). A cooperative oxygen-binding hemoglobin from Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA 96: 11223-11228 [Abstract] [Full Text]  
• Mulder, M. A., Zappe, H., Steyn, L. M. (1999). The Mycobacterium tuberculosis katG promoter region contains a novel upstream activator. Microbiology 145: 2507-2518 [Abstract] [Full Text]  
• Payton, M., Auty, R., Delgoda, R., Everett, M., Sim, E. (1999). Cloning and Characterization of Arylamine N-Acetyltransferase Genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: Increased Expression Results in Isoniazid Resistance. J. Bacteriol. 181: 1343-1347 [Abstract] [Full Text]  
• Manca, C., Paul, S., Barry, C. E. III, Freedman, V. H., Kaplan, G. (1999). Mycobacterium tuberculosis Catalase and Peroxidase Activities and Resistance to Oxidative Killing in Human Monocytes In Vitro. Infect. Immun. 67: 74-79 [Abstract] [Full Text]  
• Lyashchenko, K. P., Pollock, J. M., Colangeli, R., Gennaro, M. L. (1998). Diversity of Antigen Recognition by Serum Antibodies in Experimental Bovine Tuberculosis. Infect. Immun. 66: 5344-5349 [Abstract] [Full Text]  
• Pagán-Ramos, E., Song, J., McFalone, M., Mudd, M. H., Deretic, V. (1998). Oxidative Stress Response and Characterization of the oxyR-ahpC and furA-katG Loci in Mycobacterium marinum. J. Bacteriol. 180: 4856-4864 [Abstract] [Full Text]  
• Lyashchenko, K., Manca, C., Colangeli, R., Heijbel, A., Williams, A., Gennaro, M. L. (1998). Use of Mycobacterium tuberculosis Complex-Specific Antigen Cocktails for a Skin Test Specific for Tuberculosis. Infect. Immun. 66: 3606-3610 [Abstract] [Full Text]  
• Lyashchenko, K., Colangeli, R., Houde, M., Al Jahdali, H., Menzies, D., Gennaro, M. L. (1998). Heterogeneous Antibody Responses in Tuberculosis. Infect. Immun. 66: 3936-3940 [Abstract] [Full Text]  
• Homuth, M., Valentin-Weigand, P., Rohde, M., Gerlach, G.-F. (1998). Identification and Characterization of a Novel Extracellular Ferric Reductase from Mycobacterium paratuberculosis. Infect. Immun. 66: 710-716 [Abstract] [Full Text]  
• Nagy, J. M., Cass, A. E. G., Brown, K. A. (1997). Purification and Characterization of Recombinant Catalase-Peroxidase, Which Confers Isoniazid Sensitivity in Mycobacterium tuberculosis. J. Biol. Chem. 272: 31265-31271 [Abstract] [Full Text]  
• Marcinkeviciene, J. A., Magliozzo, R. S., Blanchard, J. S. (1995). Purification and Characterization of the Mycobacterium smegmatis Catalase-Peroxidase Involved in Isoniazid Activation. J. Biol. Chem. 270: 22290-22295 [Abstract] [Full Text]  
• Hillas, P. J., del Alba, F. S., Oyarzabal, J., Wilks, A., Ortiz de Montellano, P. R. (2000). The AhpC and AhpD Antioxidant Defense System of Mycobacterium tuberculosis. J. Biol. Chem. 275: 18801-18809 [Abstract] [Full Text]