This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brickman, T J Articles by Hackstadt, T Search for Related Content PubMed PubMed Citation Articles by Brickman, T J Articles by Hackstadt, T

research-article

Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity.

Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.

ABSTRACT

Two DNA-binding proteins with similarity to eukaryotic histone H1 have been described in Chlamydia trachomatis. In addition to the 18-kDa histone H1 homolog Hc1, elementary bodies of C. trachomatis possess an antigenically related histone H1 homolog, which we have termed Hc2, that varies in apparent molecular mass among strains. We report the molecular cloning, expression, and nucleotide sequence of the hctB gene encoding Hc2 and present evidence for in vivo DNA-binding activity of the expressed product. Expression of Hc2 in Escherichia coli induces a compaction of bacterial chromatin that is distinct from that observed upon Hc1 expression. Moreover, isolated nucleoids from Hc2-expressing E. coli exhibit markedly reduced sensitivity to DNase I. These properties of Hc2 are consistent with a postulated role in establishing the nucleoid structure of elementary bodies.

This article has been cited by other articles:

• Miura, K., Toh, H., Hirakawa, H., Sugii, M., Murata, M., Nakai, K., Tashiro, K., Kuhara, S., Azuma, Y., Shirai, M. (2008). Genome-wide Analysis of Chlamydophila pneumoniae Gene Expression at the Late Stage of Infection. DNA Res 15: 83-91 [Abstract] [Full Text]  
• Yu, H. H. Y., Kibler, D., Tan, M. (2006). In Silico Prediction and Functional Validation of {sigma}28-Regulated Genes in Chlamydia and Escherichia coli. J. Bacteriol. 188: 8206-8212 [Abstract] [Full Text]  
• Shen, L., Feng, X., Yuan, Y., Luo, X., Hatch, T. P., Hughes, K. T., Liu, J. S., Zhang, Y.-x. (2006). Selective Promoter Recognition by Chlamydial {sigma}28 Holoenzyme. J. Bacteriol. 188: 7364-7377 [Abstract] [Full Text]  
• Yu, H. H. Y., Di Russo, E. G., Rounds, M. A., Tan, M. (2006). Mutational Analysis of the Promoter Recognized by Chlamydia and Escherichia coli {sigma}28 RNA Polymerase.. J. Bacteriol. 188: 5524-5531 [Abstract] [Full Text]  
• Grieshaber, N. A., Sager, J. B., Dooley, C. A., Hayes, S. F., Hackstadt, T. (2006). Regulation of the Chlamydia trachomatis Histone H1-Like Protein Hc2 Is IspE Dependent and IhtA Independent.. J. Bacteriol. 188: 5289-5292 [Abstract] [Full Text]  
• Grieshaber, N. A., Fischer, E. R., Mead, D. J., Dooley, C. A., Hackstadt, T. (2004). From The Cover: Chlamydial histone-DNA interactions are disrupted by a metabolite in the methylerythritol phosphate pathway of isoprenoid biosynthesis. Proc. Natl. Acad. Sci. USA 101: 7451-7456 [Abstract] [Full Text]  
• Belland, R. J., Zhong, G., Crane, D. D., Hogan, D., Sturdevant, D., Sharma, J., Beatty, W. L., Caldwell, H. D. (2003). Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis. Proc. Natl. Acad. Sci. USA 100: 8478-8483 [Abstract] [Full Text]  
• Le Dantec, L., Castroviejo, M., Bove, J. M., Saillard, C. (1998). Purification, Cloning, and Preliminary Characterization of a Spiroplasma citri Ribosomal Protein with DNA Binding Capacity. J. Biol. Chem. 273: 24379-24386 [Abstract] [Full Text]