This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, L Articles by Helmann, J D Search for Related Content PubMed PubMed Citation Articles by Chen, L Articles by Helmann, J D

research-article

Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions.

Section of Microbiology, Cornell University, Ithaca, New York 14853.

ABSTRACT

We have cloned two metal-regulated genes (mrgA and mrgC) from Bacillus subtilis by using transposon Tn917-lacZ. Both were isolated as iron-repressible gene fusions, but the metal specificity and sensitivity of gene repression are distinct. Transcription of mrgA-lacZ is induced at the end of logarithmic-phase growth in minimal medium, and this induction is prevented by excess manganese, iron, cobalt, or copper. Limitation for metal ions is sufficient for mrgA-lacZ induction, since resuspension in medium lacking both manganese and iron rapidly induces transcription. Transcription of mrgC-lacZ is also induced by iron deprivation but is not repressed by added manganese or other metal ions. Expression of mrgC-lacZ and a 2,3-dihydroxybenzoic acid-based siderophore is repressed in parallel by iron, and in both cases, only iron effects repression. We have cloned and sequenced the promoter and regulatory regions of both mrgA and mrgC. Both genes are preceded by a predicted sigma A-dependent promoter element with overlapping sequences similar to the iron box consensus element for recognition by the Escherichia coli ferric uptake regulator protein (Fur). Mutation of the putative iron box for gene mrgC leads to partial derepression in iron-replete medium.

This article has been cited by other articles:

• Gabriel, S. E., Miyagi, F., Gaballa, A., Helmann, J. D. (2008). Regulation of the Bacillus subtilis yciC Gene and Insights into the DNA-Binding Specificity of the Zinc-Sensing Metalloregulator Zur. J. Bacteriol. 190: 3482-3488 [Abstract] [Full Text]  
• Lee, J.-W., Helmann, J. D. (2006). Biochemical Characterization of the Structural Zn2+ Site in the Bacillus subtilis Peroxide Sensor PerR. J. Biol. Chem. 281: 23567-23578 [Abstract] [Full Text]  
• Morikawa, K., Ohniwa, R. L., Kim, J., Maruyama, A., Ohta, T., Takeyasu, K. (2006). Bacterial nucleoid dynamics: oxidative stress response in Staphylococcus aureus. GENES CELLS 11: 409-423 [Abstract] [Full Text]  
• Wiedenheft, B., Mosolf, J., Willits, D., Yeager, M., Dryden, K. A., Young, M., Douglas, T. (2005). From The Cover: An archaeal antioxidant: Characterization of a Dps-like protein from Sulfolobus solfataricus. Proc. Natl. Acad. Sci. USA 102: 10551-10556 [Abstract] [Full Text]  
• Harvie, D. R., Vilchez, S., Steggles, J. R., Ellar, D. J. (2005). Bacillus cereus Fur regulates iron metabolism and is required for full virulence. Microbiology 151: 569-577 [Abstract] [Full Text]  
• Cao, M., Helmann, J. D. (2004). The Bacillus subtilis Extracytoplasmic-Function {sigma}X Factor Regulates Modification of the Cell Envelope and Resistance to Cationic Antimicrobial Peptides. J. Bacteriol. 186: 1136-1146 [Abstract] [Full Text]  
• Fuangthong, M., Helmann, J. D. (2003). Recognition of DNA by Three Ferric Uptake Regulator (Fur) Homologs in Bacillus subtilis. J. Bacteriol. 185: 6348-6357 [Abstract] [Full Text]  
• Steil, L., Hoffmann, T., Budde, I., Volker, U., Bremer, E. (2003). Genome-Wide Transcriptional Profiling Analysis of Adaptation of Bacillus subtilis to High Salinity. J. Bacteriol. 185: 6358-6370 [Abstract] [Full Text]  
• Helmann, J. D., Wu, M. F. W., Gaballa, A., Kobel, P. A., Morshedi, M. M., Fawcett, P., Paddon, C. (2003). The Global Transcriptional Response of Bacillus subtilis to Peroxide Stress Is Coordinated by Three Transcription Factors. J. Bacteriol. 185: 243-253 [Abstract] [Full Text]  
• Gaballa, A., Wang, T., Ye, R. W., Helmann, J. D. (2002). Functional Analysis of the Bacillus subtilis Zur Regulon. J. Bacteriol. 184: 6508-6514 [Abstract] [Full Text]  
• Ricci, S., Janulczyk, R., Bjorck, L. (2002). The Regulator PerR Is Involved in Oxidative Stress Response and Iron Homeostasis and Is Necessary for Full Virulence of Streptococcus pyogenes. Infect. Immun. 70: 4968-4976 [Abstract] [Full Text]  
• Fuangthong, M., Herbig, A. F., Bsat, N., Helmann, J. D. (2002). Regulation of the Bacillus subtilis fur and perR Genes by PerR: Not All Members of the PerR Regulon Are Peroxide Inducible. J. Bacteriol. 184: 3276-3286 [Abstract] [Full Text]  
• Hoffmann, T., Schutz, A., Brosius, M., Volker, A., Volker, U., Bremer, E. (2002). High-Salinity-Induced Iron Limitation in Bacillus subtilis. J. Bacteriol. 184: 718-727 [Abstract] [Full Text]  
• Horsburgh, M. J., Clements, M. O., Crossley, H., Ingham, E., Foster, S. J. (2001). PerR Controls Oxidative Stress Resistance and Iron Storage Proteins and Is Required for Virulence in Staphylococcus aureus. Infect. Immun. 69: 3744-3754 [Abstract] [Full Text]  
• Cabrera, G., Xiong, A., Uebel, M., Singh, V. K., Jayaswal, R. K. (2001). Molecular Characterization of the Iron-Hydroxamate Uptake System in Staphylococcus aureus. Appl. Environ. Microbiol. 67: 1001-1003 [Abstract] [Full Text]  
• Turner, M. S., Helmann, J. D. (2000). Mutations in Multidrug Efflux Homologs, Sugar Isomerases, and Antimicrobial Biosynthesis Genes Differentially Elevate Activity of the sigma X and sigma W Factors in Bacillus subtilis. J. Bacteriol. 182: 5202-5210 [Abstract] [Full Text]  
• Posey, J. E., Gherardini, F. C. (2000). Lack of a Role for Iron in the Lyme Disease Pathogen. Science 288: 1651-1653 [Abstract] [Full Text]  
• Xiong, A., Singh, V. K., Cabrera, G., Jayaswal, R. K. (2000). Molecular characterization of the ferric-uptake regulator, Fur, from Staphylococcus aureus. Microbiology 146: 659-668 [Abstract] [Full Text]  
• Bsat, N., Helmann, J. D. (1999). Interaction of Bacillus subtilis Fur (Ferric Uptake Repressor) with the dhb Operator In Vitro and In Vivo. J. Bacteriol. 181: 4299-4307 [Abstract] [Full Text]  
• de Saro, F. J. L., Yoshikawa, N., Helmann, J. D. (1999). Expression, Abundance, and RNA Polymerase Binding Properties of the delta  Factor of Bacillus subtilis. J. Biol. Chem. 274: 15953-15958 [Abstract] [Full Text]  
• Gaballa, A., Helmann, J. D. (1998). Identification of a Zinc-Specific Metalloregulatory Protein, Zur, Controlling Zinc Transport Operons in Bacillus subtilis. J. Bacteriol. 180: 5815-5821 [Abstract] [Full Text]  
• Huang, X., Fredrick, K. L., Helmann, J. D. (1998). Promoter Recognition by Bacillus subtilis sigma W: Autoregulation and Partial Overlap with the sigma X Regulon. J. Bacteriol. 180: 3765-3770 [Abstract] [Full Text]  
• Inaoka, T., Matsumura, Y., Tsuchido, T. (1998). Molecular Cloning and Nucleotide Sequence of the Superoxide Dismutase Gene and Characterization of Its Product from Bacillus subtilis. J. Bacteriol. 180: 3697-3703 [Abstract] [Full Text]  
• Bozzi, M., Mignogna, G., Stefanini, S., Barra, D., Longhi, C., Valenti, P., Chiancone, E. (1997). A Novel Non-heme Iron-binding Ferritin Related to the DNA-binding Proteins of the Dps Family in Listeria innocua. J. Biol. Chem. 272: 3259-3265 [Abstract] [Full Text]  
• Peña, M. M. O., Bullerjahn, G. S. (1995). The DpsA Protein of Synechococcus sp. Strain PCC7942 Is a DNA-binding Hemoprotein. J. Biol. Chem. 270: 22478-22482 [Abstract] [Full Text]