This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parales, R E Articles by Harwood, C S Search for Related Content PubMed PubMed Citation Articles by Parales, R E Articles by Harwood, C S

research-article

Regulation of the pcaIJ genes for aromatic acid degradation in Pseudomonas putida.

Department of Microbiology, University of Iowa, Iowa City 52242.

ABSTRACT

Six of the genes encoding enzymes of the beta-ketoadipate pathway for benzoate and 4-hydroxybenzoate degradation in Pseudomonas putida are organized into at least three separate transcriptional units. As an initial step to defining this pca regulon at the molecular level, lacZ fusions were made with the pcaI and pcaJ genes, which encode the two subunits of beta-ketoadipate:succinyl-coenzyme A transferase, the enzyme catalyzing the next-to-last step in the beta-ketoadipate pathway. Fusion analyses showed that pcaI and pcaJ constitute an operon which requires beta-ketoadipate or its nonmetabolizable analog, adipate, as well as the pcaR regulatory gene for induction. The pcaIJ promoter is likely to be a sigma 70-type promoter; it has a sigma 70-type consensus sequence and did not require the alternative sigma factor, RpoN, for induction. Deletion analysis of the promoter region of a pcaI-lacZ transcriptional fusion indicated that no specific DNA sequences upstream of the -35 region were required for full induction. This implies that the binding site for the activator protein, PcaR, is unusually close to the transcriptional start site of pcaIJ.

This article has been cited by other articles:

• Rey, F. E., Oda, Y., Harwood, C. S. (2006). Regulation of Uptake Hydrogenase and Effects of Hydrogen Utilization on Gene Expression in Rhodopseudomonas palustris.. J. Bacteriol. 188: 6143-6152 [Abstract] [Full Text]  
• MacLean, A. M., MacPherson, G., Aneja, P., Finan, T. M. (2006). Characterization of the {beta}-Ketoadipate Pathway in Sinorhizobium meliloti. Appl. Environ. Microbiol. 72: 5403-5413 [Abstract] [Full Text]  
• Buchan, A., Neidle, E. L., Moran, M. A. (2004). Diverse Organization of Genes of the {beta}-Ketoadipate Pathway in Members of the Marine Roseobacter Lineage. Appl. Environ. Microbiol. 70: 1658-1668 [Abstract] [Full Text]  
• Parales, R. E. (2004). Nitrobenzoates and Aminobenzoates Are Chemoattractants for Pseudomonas Strains. Appl. Environ. Microbiol. 70: 285-292 [Abstract] [Full Text]  
• Guvener, Z. T., McCarter, L. L. (2003). Multiple Regulators Control Capsular Polysaccharide Production in Vibrio parahaemolyticus. J. Bacteriol. 185: 5431-5441 [Abstract] [Full Text]  
• Gobel, M., Kassel-Cati, K., Schmidt, E., Reineke, W. (2002). Degradation of Aromatics and Chloroaromatics by Pseudomonas sp. Strain B13: Cloning, Characterization, and Analysis of Sequences Encoding 3-Oxoadipate:Succinyl-Coenzyme A (CoA) Transferase and 3-Oxoadipyl-CoA Thiolase. J. Bacteriol. 184: 216-223 [Abstract] [Full Text]  
• Francisco, P. B. Jr, Ogawa, N., Suzuki, K., Miyashita, K. (2001). The chlorobenzoate dioxygenase genes of Burkholderia sp. strain NK8 involved in the catabolism of chlorobenzoates. Microbiology 147: 121-133 [Abstract] [Full Text]  
• Buchan, A., Collier, L. S., Neidle, E. L., Moran, M. A. (2000). Key Aromatic-Ring-Cleaving Enzyme, Protocatechuate 3,4-Dioxygenase, in the Ecologically Important Marine Roseobacter Lineage. Appl. Environ. Microbiol. 66: 4662-4672 [Abstract] [Full Text]  
• Klemba, M., Jakobs, B., Wittich, R.-M., Pieper, D. (2000). Chromosomal Integration of tcb Chlorocatechol Degradation Pathway Genes as a Means of Expanding the Growth Substrate Range of Bacteria To Include Haloaromatics. Appl. Environ. Microbiol. 66: 3255-3261 [Abstract] [Full Text]  
• Ogawa, N., McFall, S. M., Klem, T. J., Miyashita, K., Chakrabarty, A. M. (1999). Transcriptional Activation of the Chlorocatechol Degradative Genes of Ralstonia eutropha NH9. J. Bacteriol. 181: 6697-6705 [Abstract] [Full Text]  
• Pelletier, D. A., Harwood, C. S. (1998). 2-Ketocyclohexanecarboxyl Coenzyme A Hydrolase, the Ring Cleavage Enzyme Required for Anaerobic Benzoate Degradation by Rhodopseudomonas palustris. J. Bacteriol. 180: 2330-2336 [Abstract] [Full Text]  
• Gerischer, U., Segura, A., Ornston, L. N. (1998). PcaU, a Transcriptional Activator of Genes for Protocatechuate Utilization in Acinetobacter. J. Bacteriol. 180: 1512-1524 [Abstract] [Full Text]  
• Eulberg, D., Lakner, S., Golovleva, L. A., Schlömann, M. (1998). Characterization of a Protocatechuate Catabolic Gene Cluster from Rhodococcus opacus 1CP: Evidence for a Merged Enzyme with 4-Carboxymuconolactone-Decarboxylating and 3-Oxoadipate Enol-Lactone-Hydrolyzing Activity. J. Bacteriol. 180: 1072-1081 [Abstract] [Full Text]  
• Egland, P. G., Pelletier, D. A., Dispensa, M., Gibson, J., Harwood, C. S. (1997). A cluster of bacterial genes for anaerobic benzene ring biodegradation. Proc. Natl. Acad. Sci. USA 94: 6484-6489 [Abstract] [Full Text]