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research-article

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.

Department of Cariology, School of Dentistry, University of Michigan, Ann Arbor 48109.

ABSTRACT

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

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