This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilson, I W Articles by Pittard, A J Search for Related Content PubMed PubMed Citation Articles by Wilson, I W Articles by Pittard, A J

research-article

Mutations affecting pseudoknot control of the replication of B group plasmids.

Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.

ABSTRACT

The translational initiation region of the mRNA for the replication initiation protein (RepA) of pMU720 is predicted to be sequestered in an inhibitory secondary structure designated stem-loop III. Activation of repA translation requires both the disruption of stem-loop III by ribosomes involved in the translation and termination of the leader peptide RepB and the formation of a pseudoknot, a tertiary RNA structure. Disruption of stem-loop III by site-directed mutagenesis was found to be insufficient to allow high repA expression in the absence of pseudoknot formation, indicating that the pseudoknot acts as an enhancer of repA translation. Furthermore, extending the length of the leader peptide RepB and changing the distance between the pseudoknot and repA Shine-Dalgarno sequence were found to have major effects on the translation of repA.

This article has been cited by other articles:

• Kwong, S. M., Skurray, R. A., Firth, N. (2006). Replication Control of Staphylococcal Multiresistance Plasmid pSK41: an Antisense RNA Mediates Dual-Level Regulation of Rep Expression.. J. Bacteriol. 188: 4404-4412 [Abstract] [Full Text]  
• Betteridge, T., Yang, J., Pittard, A. J., Praszkier, J. (2004). Role of RepA and DnaA Proteins in the Opening of the Origin of DNA Replication of an IncB Plasmid. J. Bacteriol. 186: 3785-3793 [Abstract] [Full Text]  
• Betteridge, T., Yang, J., Pittard, A. J., Praszkier, J. (2003). Interaction of the Initiator Protein of an IncB Plasmid with Its Origin of DNA Replication. J. Bacteriol. 185: 2210-2218 [Abstract] [Full Text]  
• Praszkier, J., Pittard, A. J. (2002). Pseudoknot-Dependent Translational Coupling in repBA Genes of the IncB Plasmid pMU720 Involves Reinitiation. J. Bacteriol. 184: 5772-5780 [Abstract] [Full Text]  
• Kolb, F. A., Westhof, E., Ehresmann, C., Ehresmann, B., Wagner, E. G. H., Romby, P. (2001). Bulged residues promote the progression of a loop-loop interaction to a stable and inhibitory antisense-target RNA complex. Nucleic Acids Res 29: 3145-3153 [Abstract] [Full Text]  
• Praszkier, J., Murthy, S., Pittard, A. J. (2000). Effect of CIS on Activity in trans of the Replication Initiator Protein of an IncB Plasmid. J. Bacteriol. 182: 3972-3980 [Abstract] [Full Text]  
• Asano, K., Mizobuchi, K. (2000). Structural Analysis of Late Intermediate Complex Formed between Plasmid ColIb-P9 Inc RNA and Its Target RNA. HOW DOES A SINGLE ANTISENSE RNA REPRESS TRANSLATION OF TWO GENES AT DIFFERENT RATES?. J. Biol. Chem. 275: 1269-1274 [Abstract] [Full Text]  
• Praszkier, J., Pittard, A. J. (1999). Role of CIS in Replication of an IncB Plasmid. J. Bacteriol. 181: 2765-2772 [Abstract] [Full Text]  
• Athanasopoulos, V., Praszkier, J., Pittard, A. J. (1999). Analysis of Elements Involved in Pseudoknot-Dependent Expression and Regulation of the repA Gene of an IncL/M Plasmid. J. Bacteriol. 181: 1811-1819 [Abstract] [Full Text]  
• Asano, K., Mizobuchi, K. (1998). An RNA Pseudoknot as the Molecular Switch for Translation of the repZ Gene Encoding the Replication Initiator of IncIalpha Plasmid ColIb-P9. J. Biol. Chem. 273: 11815-11825 [Abstract] [Full Text]  
• Malmgren, C., Wagner, E. G. H., Ehresmann, C., Ehresmann, B., Romby, P. (1997). Antisense RNA Control of Plasmid R1 Replication. THE DOMINANT PRODUCT OF THE ANTISENSE RNA-mRNA BINDING IS NOT A FULL RNA DUPLEX. J. Biol. Chem. 272: 12508-12512 [Abstract] [Full Text]