This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, K S Articles by Walker, J R Search for Related Content PubMed PubMed Citation Articles by Chen, K S Articles by Walker, J R

research-article

Expression of the Escherichia coli dnaX gene.

Department of Microbiology, University of Texas, Austin 78712-1095.

ABSTRACT

The Escherichia coli dnaX gene encodes both the tau and gamma subunits of DNA polymerase III. This gene is located immediately downstream of the adenine salvage gene apt and upstream of orf12-recR, htpG, and adk. The last three are involved in recombination, heat shock, and nucleotide biosynthesis, respectively. apt, dnaX, and orf12-recR all have separate promoters, and the first two are expressed predominantly from those separate promoters. However, use of an RNase E temperature-sensitive mutant allowed the detection of lesser amounts of polycistronic messengers extending from both the apt and dnaX promoters through htpG. Interestingly, transcription of the weak dnaX promoter is stimulated 4- to 10-fold by a sequence contained entirely within the dnaX reading frame. This region has been localized; at least a portion of the sequence (and perhaps the entire sequence) is located within a 31-bp region downstream of the dnaX promoter.

This article has been cited by other articles:

• Babb, K., Bykowski, T., Riley, S. P., Miller, M. C., DeMoll, E., Stevenson, B. (2006). Borrelia burgdorferi EbfC, a Novel, Chromosomally Encoded Protein, Binds Specific DNA Sequences Adjacent to erp Loci on the Spirochete's Resident cp32 Prophages.. J. Bacteriol. 188: 4331-4339 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]