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J Bacteriol. 1993 October; 175(20): 6717-6720

research-article

Identification of an intracellular pyrimidine-specific endoribonuclease from Bacillus subtilis.

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.

ABSTRACT

Two intracellular RNases which were easily separated by fractionation on strong anion- or cation-exchange resins were identified from Bacillus subtilis. One cleaved any phosphodiester bond, while the second cleaved only pyrimidine-N bonds. The enzyme with pyrimidine-N specificity was approximately 15 kDa, had a pH optimum of approximately 6.2, degraded C-C bonds approximately 10 times faster than U-U bonds, and was completely inactive against single-stranded DNA. The enzyme is called RNase C and may be the first reported broad-specificity endoribonuclease from B. subtilis.

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