This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, M H Articles by Hatfull, G F Search for Related Content PubMed PubMed Citation Articles by Lee, M H Articles by Hatfull, G F

research-article

Mycobacteriophage L5 integrase-mediated site-specific integration in vitro.

Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.

ABSTRACT

Mycobacteriophage L5, a temperate phage of the mycobacteria, forms stable lysogens in Mycobacterium smegmatis via site-specific integration of the phage genome. Recombination occurs within specific phage and bacterial attachment sites and is catalyzed by the phage-encoded integrase protein in vivo. We describe here the overexpression and purification of L5 integrase and its ability to mediate integrative recombination in vitro. We find that L5 integrase-mediated recombination is greatly stimulated by extracts of M. smegmatis but not by Escherichia coli extracts, purified E. coli integration host factor, or purified HU, indicating the presence of a novel mycobacterial integration host factor.

This article has been cited by other articles:

• Bryan, A., Roesch, P., Davis, L., Moritz, R., Pellett, S., Welch, R. A. (2006). Regulation of Type 1 Fimbriae by Unlinked FimB- and FimE-Like Recombinases in Uropathogenic Escherichia coli Strain CFT073. Infect. Immun. 74: 1072-1083 [Abstract] [Full Text]  
• Saviola, B., Bishai, W. R. (2004). Method to integrate multiple plasmids into the mycobacterial chromosome. Nucleic Acids Res 32: e11-e11 [Abstract] [Full Text]  
• Chacon, O., Feng, Z., Harris, N. B., Caceres, N. E., Adams, L. G., Barletta, R. G. (2002). Mycobacterium smegmatisD-Alanine Racemase Mutants Are Not Dependent on D-Alanine for Growth. Antimicrob. Agents Chemother. 46: 47-54 [Abstract] [Full Text]  
• Esposito, D., Thrower, J. S., Scocca, J. J. (2001). Protein and DNA requirements of the bacteriophage HP1 recombination system: a model for intasome formation. Nucleic Acids Res 29: 3955-3964 [Abstract] [Full Text]  
• Peña, C. E. A., Kahlenberg, J. M., Hatfull, G. F. (1999). Protein-DNA Complexes in Mycobacteriophage L5 Integrative Recombination. J. Bacteriol. 181: 454-461 [Abstract] [Full Text]  
• Pedulla, M. L., Hatfull, G. F. (1998). Characterization of the mIHF Gene of Mycobacterium smegmatis. J. Bacteriol. 180: 5473-5477 [Abstract] [Full Text]