This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Strobel, S M Articles by Bassford, P J Search for Related Content PubMed PubMed Citation Articles by Strobel, S M Articles by Bassford, P J, Jr

research-article

Regions of maltose-binding protein that influence SecB-dependent and SecA-dependent export in Escherichia coli.

Department of Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill 27599-7290.

ABSTRACT

In Escherichia coli, the efficient export of maltose-binding protein (MBP) is dependent on the chaperone SecB, whereas export of ribose-binding protein (RBP) is SecB independent. To localize the regions of MBP involved in interaction with SecB, hybrids between MBP and RBP in SecB mutant cells were constructed and analyzed. One hybrid consisted of the signal peptide and first third of the mature moiety of MBP, followed by the C-terminal two-thirds of RBP (MBP-RBP112). This hybrid was dependent upon SecB for its efficient export and exhibited a strong export defect in secA mutant cells. A hybrid between RBP and MBP with the same fusion point was also constructed (RBP-MBP116). The RBP-MBP116 hybrid remained SecB independent and only exhibited a partial export defect in secA mutant cells. In addition, MBP species with specific alterations in the early mature region were less dependent on SecB for their efficient export. The export of these altered MBP species was also less affected in secA mutant cells and in cells treated with sodium azide. These results present additional evidence for the targeting role of SecB.

This article has been cited by other articles:

• Kim, J., Kendall, D. A. (1998). Identification of a Sequence Motif That Confers SecB Dependence on a SecB-Independent Secretory Protein In Vivo. J. Bacteriol. 180: 1396-1401 [Abstract] [Full Text]  
• Boos, W., Shuman, H. (1998). Maltose/Maltodextrin System of Escherichia coli: Transport, Metabolism, and Regulation. Microbiol. Mol. Biol. Rev. 62: 204-229 [Abstract] [Full Text]