This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grundy, F J Articles by Henkin, T M Search for Related Content PubMed PubMed Citation Articles by Grundy, F J Articles by Henkin, T M

research-article

Regulation of the Bacillus subtilis acetate kinase gene by CcpA.

Department of Biochemistry and Molecular Biology, Albany Medical College, New York 12208.

ABSTRACT

The Bacillus subtilis gene encoding acetate kinase was identified on the basis of sequence similarity to the Escherichia coli ackA gene and to a second E. coli gene closely related to ackA. Insertional inactivation of this region of the B. subtilis chromosome resulted in the disappearance of acetate kinase enzyme activity in cell extracts. The ackA gene was mapped to a site close to the ccpA gene, at 263 degrees. The transcriptional start site for B. subtilis ackA was located 90 bp upstream from the start of the coding region, and expression was increased by growth in the presence of excess glucose. Growth of the AckA- mutant was inhibited by glucose, suggesting that acetate kinase is important for excretion of excess carbohydrate. The stimulation of ackA expression by glucose was blocked in a CcpA- mutant, indicating that CcpA, which is required for glucose repression of certain carbon source utilization genes, including amyE, may also be involved in activation of carbon excretion pathways. Two sequences resembling the amyO operator site were identified upstream of the ackA promoter; removal of this region resulted in loss of glucose activation of ackA expression.

This article has been cited by other articles:

• Kinkel, T. L., McIver, K. S. (2008). CcpA-Mediated Repression of Streptolysin S Expression and Virulence in the Group A Streptococcus. Infect. Immun. 76: 3451-3463 [Abstract] [Full Text]  
• Almengor, A. C., Kinkel, T. L., Day, S. J., McIver, K. S. (2007). The Catabolite Control Protein CcpA Binds to Pmga and Influences Expression of the Virulence Regulator Mga in the Group A Streptococcus. J. Bacteriol. 189: 8405-8416 [Abstract] [Full Text]  
• Horstmann, N., Seidel, G., Aung-Hilbrich, L.-M., Hillen, W. (2007). Residues His-15 and Arg-17 of HPr Participate Differently in Catabolite Signal Processing via CcpA. J. Biol. Chem. 282: 1175-1182 [Abstract] [Full Text]  
• Lorca, G. L., Chung, Y. J., Barabote, R. D., Weyler, W., Schilling, C. H., Saier, M. H. Jr (2005). Catabolite Repression and Activation in Bacillus subtilis: Dependency on CcpA, HPr, and HprK. J. Bacteriol. 187: 7826-7839 [Abstract] [Full Text]  
• Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  
• Merritt, J., Qi, F., Shi, W. (2005). A unique nine-gene comY operon in Streptococcus mutans. Microbiology 151: 157-166 [Abstract] [Full Text]  
• Gerstmeir, R., Cramer, A., Dangel, P., Schaffer, S., Eikmanns, B. J. (2004). RamB, a Novel Transcriptional Regulator of Genes Involved in Acetate Metabolism of Corynebacterium glutamicum. J. Bacteriol. 186: 2798-2809 [Abstract] [Full Text]  
• Grundy, F. J., Lehman, S. C., Henkin, T. M. (2003). The L box regulon: Lysine sensing by leader RNAs of bacterial lysine biosynthesis genes. Proc. Natl. Acad. Sci. USA 100: 12057-12062 [Abstract] [Full Text]  
• Ramstrom, H., Sanglier, S., Leize-Wagner, E., Philippe, C., Van Dorsselaer, A., Haiech, J. (2003). Properties and Regulation of the Bifunctional Enzyme HPr Kinase/Phosphatase in Bacillus subtilis. J. Biol. Chem. 278: 1174-1185 [Abstract] [Full Text]  
• Giammarinaro, P., Paton, J. C. (2002). Role of RegM, a Homologue of the Catabolite Repressor Protein CcpA, in the Virulence of Streptococcus pneumoniae. Infect. Immun. 70: 5454-5461 [Abstract] [Full Text]  
• Grundy, F. J., Winkler, W. C., Henkin, T. M. (2002). tRNA-mediated transcription antitermination in vitro: Codon-anticodon pairing independent of the ribosome. Proc. Natl. Acad. Sci. USA 99: 11121-11126 [Abstract] [Full Text]  
• Moir-Blais, T. R., Grundy, F. J., Henkin, T. M. (2001). Transcriptional Activation of the Bacillus subtilis ackA Promoter Requires Sequences Upstream of the CcpA Binding Site. J. Bacteriol. 183: 2389-2393 [Abstract] [Full Text]  
• van den Bogaard, P. T. C., Kleerebezem, M., Kuipers, O. P., de Vos, W. M. (2000). Control of Lactose Transport, beta -Galactosidase Activity, and Glycolysis by CcpA in Streptococcus thermophilus: Evidence for Carbon Catabolite Repression by a Non-Phosphoenolpyruvate-Dependent Phosphotransferase System Sugar. J. Bacteriol. 182: 5982-5989 [Abstract] [Full Text]  
• Leboeuf, C., Leblanc, L., Auffray, Y., Hartke, A. (2000). Characterization of the ccpA Gene of Enterococcus faecalis: Identification of Starvation-Inducible Proteins Regulated by CcpA. J. Bacteriol. 182: 5799-5806 [Abstract] [Full Text]  
• Turinsky, A. J., Moir-Blais, T. R., Grundy, F. J., Henkin, T. M. (2000). Bacillus subtilis ccpA Gene Mutants Specifically Defective in Activation of Acetoin Biosynthesis. J. Bacteriol. 182: 5611-5614 [Abstract] [Full Text]  
• Miwa, Y., Nakata, A., Ogiwara, A., Yamamoto, M., Fujita, Y. (2000). Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis. Nucleic Acids Res 28: 1206-1210 [Abstract] [Full Text]  
• Presecan-Siedel, E., Galinier, A., Longin, R., Deutscher, J., Danchin, A., Glaser, P., Martin-Verstraete, I. (1999). Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in Bacillus subtilis. J. Bacteriol. 181: 6889-6897 [Abstract] [Full Text]  
• Tobisch, S., Zühlke, D., Bernhardt, J., Stülke, J., Hecker, M. (1999). Role of CcpA in Regulation of the Central Pathways of Carbon Catabolism in Bacillus subtilis. J. Bacteriol. 181: 6996-7004 [Abstract] [Full Text]  
• Ward, D. E., Ross, R. P., van der Weijden, C. C., Snoep, J. L., Claiborne, A. (1999). Catabolism of Branched-Chain alpha -Keto Acids in Enterococcus faecalis: the bkd Gene Cluster, Enzymes, and Metabolic Route. J. Bacteriol. 181: 5433-5442 [Abstract] [Full Text]  
• Turinsky, A. J., Grundy, F. J., Kim, J.-H., Chambliss, G. H., Henkin, T. M. (1998). Transcriptional Activation of the Bacillus subtilis ackA Gene Requires Sequences Upstream of the Promoter. J. Bacteriol. 180: 5961-5967 [Abstract] [Full Text]