This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sanderson, K E Articles by Hessel, A Search for Related Content PubMed PubMed Citation Articles by Sanderson, K E Articles by Hessel, A

research-article

Location of IS200 on the genomic cleavage map of Salmonella typhimurium LT2.

Department of Biological Sciences, University of Calgary, Alberta, Canada.

ABSTRACT

Locations of six Tn10s, closely linked to each of the six IS200s on the genomic cleavage map of Salmonella typhimurium LT2, were determined by digestion with XbaI and BlnI and separation of the fragments by pulsed-field gel electrophoresis; the locations were then further defined by P22-mediated joint transduction. The orientation of each IS200 with respect to its linked Tn10 was determined by Southern blotting. The locations of IS200-I, IS200-III, and IS200-V were confirmed to be close to sufD, melB, and purC, as previously indicated. IS200-II is jointly transduced with cysG. IS200-IV is near fliA; the linked Tn10 is inserted in fli, making the strain nonmotile. IS200-VI is jointly transduced with aspC but not with aroA. IS200 is transposed to a seventh site in some strains, while remaining in the other six locations described above. These data indicate that genome analysis by pulsed-field gel electrophoresis can locate the positions of Tn10s with accuracy sufficient to predict P22-mediated joint transduction.

This article has been cited by other articles:

• Liu, G.-R., Liu, W.-Q., Johnston, R. N., Sanderson, K. E., Li, S.-X., Liu, S.-L. (2006). Genome Plasticity and ori-ter Rebalancing in Salmonella typhi. Mol Biol Evol 23: 365-371 [Abstract] [Full Text]  
• Liu, G.-R., Edwards, K., Eisenstark, A., Fu, Y.-M., Liu, W.-Q., Sanderson, K. E., Johnston, R. N., Liu, S.-L. (2003). Genomic Diversification among Archival Strains of Salmonella enterica Serovar Typhimurium LT7. J. Bacteriol. 185: 2131-2142 [Abstract] [Full Text]  
• Alokam, S., Liu, S.-L., Said, K., Sanderson, K. E. (2002). Inversions over the Terminus Region in Salmonella and Escherichia coli: IS200s as the Sites of Homologous Recombination Inverting the Chromosome of Salmonella enterica Serovar Typhi. J. Bacteriol. 184: 6190-6197 [Abstract] [Full Text]  
• Echeita, M. A., Herrera, S., Usera, M. A. (2001). Atypical, fljB-Negative Salmonella enterica subsp. enterica Strain of Serovar 4,5,12:i:{-} Appears To Be a Monophasic Variant of Serovar Typhimurium. J. Clin. Microbiol. 39: 2981-2983 [Abstract] [Full Text]  
• Kofoid, E., Rappleye, C., Stojiljkovic, I., Roth, J. (1999). The 17-Gene Ethanolamine (eut) Operon of Salmonella typhimurium Encodes Five Homologues of Carboxysome Shell Proteins. J. Bacteriol. 181: 5317-5329 [Abstract] [Full Text]