This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paradis, F W Articles by Forsberg, C W Search for Related Content PubMed PubMed Citation Articles by Paradis, F W Articles by Forsberg, C W

research-article

The xynC gene from Fibrobacter succinogenes S85 codes for a xylanase with two similar catalytic domains.

Department of Microbiology, University of Guelph, Ontario, Canada.

ABSTRACT

The xynC gene of Fibrobacter succinogenes S85 codes for a 66.4-kDa xylanase which consists of three distinct domains separated by two flexible regions rich in serine residues. Domains A and B of XynC code for catalytic domains with 56.5% identity and 9.6% similarity with each other, and both domains share homology with xylanases of Ruminococcus flavefaciens, Neocallimastix patriciarum, Clostridium acetobutylicum, Bacillus pumilus, Bacillus subtilis, and Bacillus circulans. More than 88% of the xylanase activity of Escherichia coli cells carrying the original 13-kb recombinant plasmid was released from intact cells by cold water washes. The major products of hydrolysis of xylan by both domains were xylose and xylobiose, indicating that the xynC gene product exhibits catalytic properties similar to those of the XynA xylanases from R. flavefaciens and N. patriciarum. So far, these features are not shared broadly with bacteria from other environments and may indicate specific selection for this domain structure in the highly competitive environment of the rumen.

This article has been cited by other articles:

• Qi, M., Nelson, K. E., Daugherty, S. C., Nelson, W. C., Hance, I. R., Morrison, M., Forsberg, C. W. (2008). Genomic Differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, Identified by Suppression Subtractive Hybridization. Appl. Environ. Microbiol. 74: 987-993 [Abstract] [Full Text]  
• Matulova, M., Nouaille, R., Capek, P., Pean, M., Forano, E., Delort, A.-M. (2005). Degradation of Wheat Straw by Fibrobacter succinogenes S85: a Liquid- and Solid-State Nuclear Magnetic Resonance Study. Appl. Environ. Microbiol. 71: 1247-1253 [Abstract] [Full Text]  
• Howard, M. B., Ekborg, N. A., Taylor, L. E. II, Weiner, R. M., Hutcheson, S. W. (2004). Chitinase B of "Microbulbifer degradans" 2-40 Contains Two Catalytic Domains with Different Chitinolytic Activities. J. Bacteriol. 186: 1297-1303 [Abstract] [Full Text]  
• Marrone, L., McAllister, K. A., Clarke, A. J. (2000). Characterization of function and activity of domains A, B and C of xylanase C from Fibrobacter succinogenes S85. Protein Eng Des Sel 13: 593-601 [Abstract] [Full Text]  
• Morris, D. D., Gibbs, M. D., Chin, C. W. J., Koh, M.-H., Wong, K. K. Y., Allison, R. W., Nelson, P. J., Bergquist, P. L. (1998). Cloning of the xynB Gene from Dictyoglomus thermophilum Rt46B.1 and Action of the Gene Product on Kraft Pulp. Appl. Environ. Microbiol. 64: 1759-1765 [Abstract] [Full Text]