This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lange, R Articles by Hengge-Aronis, R Search for Related Content PubMed PubMed Citation Articles by Lange, R Articles by Hengge-Aronis, R

research-article

Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli.

Department of Biology, University of Konstanz, Germany.

ABSTRACT

osmY (csi-5) is a representative of a large group of sigma s-dependent genes in Escherichia coli that exhibit both stationary-phase induction and osmotic regulation. A chromosomal transcriptional lacZ fusion (csi-5::lacZ) was used to study the regulation of osmY. We show here that in addition to sigma s, the global regulators Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex (cAMP-CRP), and integration host factor (IHF) are involved in the control of osmY. All three regulators negatively modulate the expression of osmY, and they act independently from sigma s. Stationary-phase induction of osmY in minimal medium can be explained by stimulation by sigma s combined with a relief of Lrp repression. Stationary-phase induction of osmY in rich medium is mediated by the combined action of sigma s, Lrp, cAMP-CRP, and IHF, with the latter three proteins acting as transition state regulators. The transcriptional start site of osmY was determined and revealed an mRNA with an unusual long nontranslated leader of 244 nucleotides. The regulatory region is characterized by a sigma 70-like -10 promoter region and contains potential binding sites for Lrp, CRP, and IHF. Whereas sigma s, Lrp, CRP, and IHF are clearly involved in stationary-phase induction, none of these regulators is essential for osmotic regulation of osmY.

This article has been cited by other articles:

• Lawrenz, M. B., Miller, V. L. (2007). Comparative Analysis of the Regulation of rovA from the Pathogenic Yersiniae. J. Bacteriol. 189: 5963-5975 [Abstract] [Full Text]  
• Lelong, C., Aguiluz, K., Luche, S., Kuhn, L., Garin, J., Rabilloud, T., Geiselmann, J. (2007). The Crl-RpoS Regulon of Escherichia coli. Mol. Cell. Proteomics 6: 648-659 [Abstract] [Full Text]  
• Han, M.-J., Lee, S. Y. (2006). The Escherichia coli Proteome: Past, Present, and Future Prospects. Microbiol. Mol. Biol. Rev. 70: 362-439 [Abstract] [Full Text]  
• Weber, H., Polen, T., Heuveling, J., Wendisch, V. F., Hengge, R. (2005). Genome-Wide Analysis of the General Stress Response Network in Escherichia coli: {sigma}S-Dependent Genes, Promoters, and Sigma Factor Selectivity. J. Bacteriol. 187: 1591-1603 [Abstract] [Full Text]  
• Caimano, M. J., Eggers, C. H., Hazlett, K. R. O., Radolf, J. D. (2004). RpoS Is Not Central to the General Stress Response in Borrelia burgdorferi but Does Control Expression of One or More Essential Virulence Determinants. Infect. Immun. 72: 6433-6445 [Abstract] [Full Text]  
• Lacour, S., Landini, P. (2004). {sigma}S-Dependent Gene Expression at the Onset of Stationary Phase in Escherichia coli: Function of {sigma}S-Dependent Genes and Identification of Their Promoter Sequences. J. Bacteriol. 186: 7186-7195 [Abstract] [Full Text]  
• Eggers, C. H., Caimano, M. J., Radolf, J. D. (2004). Analysis of Promoter Elements Involved in the Transcriptional Initiation of RpoS-Dependent Borrelia burgdorferi Genes. J. Bacteriol. 186: 7390-7402 [Abstract] [Full Text]  
• Motin, V. L., Georgescu, A. M., Fitch, J. P., Gu, P. P., Nelson, D. O., Mabery, S. L., Garnham, J. B., Sokhansanj, B. A., Ott, L. L., Coleman, M. A., Elliott, J. M., Kegelmeyer, L. M., Wyrobek, A. J., Slezak, T. R., Brubaker, R. R., Garcia, E. (2004). Temporal Global Changes in Gene Expression during Temperature Transition in Yersinia pestis. J. Bacteriol. 186: 6298-6305 [Abstract] [Full Text]  
• Ma, Z., Richard, H., Foster, J. W. (2003). pH-Dependent Modulation of Cyclic AMP Levels and GadW-Dependent Repression of RpoS Affect Synthesis of the GadX Regulator and Escherichia coli Acid Resistance. J. Bacteriol. 185: 6852-6859 [Abstract] [Full Text]  
• Chen, G., Patten, C. L., Schellhorn, H. E. (2003). Controlled Expression of an rpoS Antisense RNA Can Inhibit RpoS Function in Escherichia coli. Antimicrob. Agents Chemother. 47: 3485-3493 [Abstract] [Full Text]  
• Krin, E., Laurent-Winter, C., Bertin, P. N., Danchin, A., Kolb, A. (2003). Transcription Regulation Coupling of the Divergent argG and metY Promoters in Escherichia coli K-12. J. Bacteriol. 185: 3139-3146 [Abstract] [Full Text]  
• Tani, T. H., Khodursky, A., Blumenthal, R. M., Brown, P. O., Matthews, R. G. (2002). Adaptation to famine: A family of stationary-phase genes revealed by microarray analysis. Proc. Natl. Acad. Sci. USA 99: 13471-13476 [Abstract] [Full Text]  
• Sánchez-SanMartín, C., Bustamante, V. H., Calva, E., Puente, J. L. (2001). Transcriptional Regulation of the orf19 Gene and the tir-cesT-eae Operon of Enteropathogenic Escherichia coli. J. Bacteriol. 183: 2823-2833 [Abstract] [Full Text]  
• Phadtare, S., Inouye, M. (2001). Role of CspC and CspE in Regulation of Expression of RpoS and UspA, the Stress Response Proteins in Escherichia coli. J. Bacteriol. 183: 1205-1214 [Abstract] [Full Text]  
• Davey, M. E., de Bruijn, F. J. (2000). A Homologue of the Tryptophan-Rich Sensory Protein TspO and FixL Regulate a Novel Nutrient Deprivation-Induced Sinorhizobium meliloti Locus. Appl. Environ. Microbiol. 66: 5353-5359 [Abstract] [Full Text]  
• Indest, K. J., Philipp, M. T. (2000). DNA-Binding Proteins Possibly Involved in Regulation of the Post-Logarithmic-Phase Expression of Lipoprotein P35 in Borrelia burgdorferi. J. Bacteriol. 182: 522-525 [Abstract] [Full Text]  
• Wood, J. M. (1999). Osmosensing by Bacteria: Signals and Membrane-Based Sensors. Microbiol. Mol. Biol. Rev. 63: 230-262 [Abstract] [Full Text]  
• Fischer, D., Teich, A., Neubauer, P., Hengge-Aronis, R. (1998). The General Stress Sigma Factor sigma S of Escherichia coli Is Induced during Diauxic Shift from Glucose to Lactose. J. Bacteriol. 180: 6203-6206 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Peekhaus, N., Conway, T. (1998). Positive and Negative Transcriptional Regulation of the Escherichia coli Gluconate Regulon Gene gntT by GntR and the Cyclic AMP (cAMP)-cAMP Receptor Protein Complex. J. Bacteriol. 180: 1777-1785 [Abstract] [Full Text]  
• Germer, J., Muffler, A., Hengge-Aronis, R. (1998). Trehalose Is Not Relevant for In Vivo Activity of sigma S-Containing RNA Polymerase in Escherichia coli. J. Bacteriol. 180: 1603-1606 [Abstract] [Full Text]  
• Van Dyk, T. K., Ayers, B. L., Morgan, R. W., Larossa, R. A. (1998). Constricted Flux through the Branched-Chain Amino Acid Biosynthetic Enzyme Acetolactate Synthase Triggers Elevated Expression of Genes Regulated by rpoS and Internal Acidification. J. Bacteriol. 180: 785-792 [Abstract] [Full Text]  
• Lange, R, Hengge-Aronis, R (1994). The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability.. Genes Dev. 8: 1600-1612 [Abstract]