This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Saffarini, D A Articles by Nealson, K H Search for Related Content PubMed PubMed Citation Articles by Saffarini, D A Articles by Nealson, K H

research-article

Sequence and genetic characterization of etrA, an fnr analog that regulates anaerobic respiration in Shewanella putrefaciens MR-1.

Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53204.

ABSTRACT

An electron transport regulatory gene, etrA, has been isolated and characterized from the obligate respiratory bacterium Shewanella putrefaciens MR-1. The deduced amino acid sequence of etrA (EtrA) shows a high degree of identity to both the Fnr of Escherichia coli (73.6%) and the analogous protein (ANR) of Pseudomonas aeruginosa (50.8%). The four active cysteine residues of Fnr are conserved in EtrA, and the amino acid sequence of the DNA-binding domains of the two proteins are identical. Further, S. putrefaciens etrA is able to complement an fnr mutant of E. coli. In contrast to fnr, there is no recognizable Fnr box upstream of the etrA sequence. Gene replacement etrA mutants of MR-1 were deficient in growth on nitrite, thiosulfate, sulfite, trimethylamine-N-oxide, dimethyl sulfoxide, Fe(III), and fumarate, suggesting that EtrA is involved in the regulation of the corresponding reductase genes. However, the mutants were all positive for reduction of and growth on nitrate and Mn(IV), indicating that EtrA is not involved in the regulation of these two systems. Southern blots of S. putrefaciens DNA with use of etrA as a probe revealed the expected etrA bands and a second set of hybridization signals whose genetic and functional properties remain to be determined.

This article has been cited by other articles:

• Thormann, K. M., Saville, R. M., Shukla, S., Spormann, A. M. (2005). Induction of Rapid Detachment in Shewanella oneidensis MR-1 Biofilms. J. Bacteriol. 187: 1014-1021 [Abstract] [Full Text]  
• Wan, X.-F., VerBerkmoes, N. C., McCue, L. A., Stanek, D., Connelly, H., Hauser, L. J., Wu, L., Liu, X., Yan, T., Leaphart, A., Hettich, R. L., Zhou, J., Thompson, D. K. (2004). Transcriptomic and Proteomic Characterization of the Fur Modulon in the Metal-Reducing Bacterium Shewanella oneidensis. J. Bacteriol. 186: 8385-8400 [Abstract] [Full Text]  
• Saffarini, D. A., Schultz, R., Beliaev, A. (2003). Involvement of Cyclic AMP (cAMP) and cAMP Receptor Protein in Anaerobic Respiration of Shewanella oneidensis. J. Bacteriol. 185: 3668-3671 [Abstract] [Full Text]  
• Beliaev, A. S., Thompson, D. K., Fields, M. W., Wu, L., Lies, D. P., Nealson, K. H., Zhou, J. (2002). Microarray Transcription Profiling of a Shewanella oneidensis etrA Mutant. J. Bacteriol. 184: 4612-4616 [Abstract] [Full Text]  
• Thompson, D. K., Beliaev, A. S., Giometti, C. S., Tollaksen, S. L., Khare, T., Lies, D. P., Nealson, K. H., Lim, H., Yates, J. III, Brandt, C. C., Tiedje, J. M., Zhou, J. (2002). Transcriptional and Proteomic Analysis of a Ferric Uptake Regulator (Fur) Mutant of Shewanella oneidensis: Possible Involvement of Fur in Energy Metabolism, Transcriptional Regulation, and Oxidative Stress. Appl. Environ. Microbiol. 68: 881-892 [Abstract] [Full Text]  
• Maier, T. M., Myers, C. R. (2001). Isolation and Characterization of a Shewanella putrefaciens MR-1 Electron Transport Regulator etrA Mutant: Reassessment of the Role of EtrA. J. Bacteriol. 183: 4918-4926 [Abstract] [Full Text]  
• Myers, J. M., Myers, C. R. (2000). Role of the Tetraheme Cytochrome CymA in Anaerobic Electron Transport in Cells of Shewanella putrefaciens MR-1 with Normal Levels of Menaquinone. J. Bacteriol. 182: 67-75 [Abstract] [Full Text]