This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kündig, C Articles by Göttfert, M Search for Related Content PubMed PubMed Citation Articles by Kündig, C Articles by Göttfert, M

research-article

Correlated physical and genetic map of the Bradyrhizobium japonicum 110 genome.

Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.

ABSTRACT

We describe a compilation of 79 known genes of Bradyrhizobium japonicum 110, 63 of which were placed on a correlated physical and genetic map of the chromosome. Genomic DNA was restricted with enzymes PacI, PmeI, and SwaI, which yielded two, five, and nine fragments, respectively. Linkage of some of the fragments was established by performing Southern blot hybridization experiments. For probes we used isolated, labelled fragments that were produced either by PmeI or by SwaI. Genes were mapped on individual restriction fragments by performing gene-directed mutagenesis. The principle of this method was to introduce recognition sites for all three restriction enzymes mentioned above into or very near the desired gene loci. Pulsed-field gel electrophoresis of restricted mutant DNA then resulted in an altered fragment pattern compared with wild-type DNA. This allowed us to identify overlapping fragments and to determine the exact position of any selected gene locus. The technique was limited only by the accuracy of the fragment size estimates. After linkage of all of the restriction fragments we concluded that the B. japonicum genome consists of a single, circular chromosome that is approximately 8,700 kb long. Genes directly concerned with nodulation and symbiotic nitrogen fixation are clustered in a chromosomal section that is about 380 kb long.

This article has been cited by other articles:

• Wei, M., Yokoyama, T., Minamisawa, K., Mitsui, H., Itakura, M., Kaneko, T., Tabata, S., Saeki, K., Omori, H., Tajima, S., Uchiumi, T., Abe, M., Ohwada, T. (2008). Soybean Seed Extracts Preferentially Express Genomic Loci of Bradyrhizobium japonicum in the Initial Interaction with Soybean, Glycine max (L.) Merr. DNA Res 0: dsn012v2-14 [Abstract] [Full Text]  
• Barcellos, F. G., Menna, P., da Silva Batista, J. S., Hungria, M. (2007). Evidence of Horizontal Transfer of Symbiotic Genes from a Bradyrhizobium japonicum Inoculant Strain to Indigenous Diazotrophs Sinorhizobium (Ensifer) fredii and Bradyrhizobium elkanii in a Brazilian Savannah Soil. Appl. Environ. Microbiol. 73: 2635-2643 [Abstract] [Full Text]  
• Berg, O. G., Kurland, C. G. (2002). Evolution of Microbial Genomes: Sequence Acquisition and Loss. Mol Biol Evol 19: 2265-2276 [Abstract] [Full Text]  
• Mesa, S., Velasco, L., Manzanera, M. E., Delgado, M. J., Bedmar, E. J. (2002). Characterization of the norCBQD genes, encoding nitric oxide reductase, in the nitrogen fixing bacterium Bradyrhizobium japonicum. Microbiology 148: 3553-3560 [Abstract] [Full Text]  
• Parker, M. A., Lafay, B., Burdon, J. J., van Berkum, P. (2002). Conflicting phylogeographic patterns in rRNA and nifD indicate regionally restricted gene transfer in Bradyrhizobium. Microbiology 148: 2557-2565 [Abstract] [Full Text]  
• Yasuta, T., Okazaki, S., Mitsui, H., Yuhashi, K.-I., Ezura, H., Minamisawa, K. (2001). DNA Sequence and Mutational Analysis of Rhizobitoxine Biosynthesis Genes in Bradyrhizobium elkanii. Appl. Environ. Microbiol. 67: 4999-5009 [Abstract] [Full Text]  
• Tomkins, J. P., Wood, T. C., Stacey, M. G., Loh, J. T., Judd, A., Goicoechea, J. L., Stacey, G., Sadowsky, M. J., Wing, R. A. (2001). A Marker-Dense Physical Map of the Bradyrhizobium japonicum Genome. Genome Res. 11: 1434-1440 [Abstract] [Full Text]  
• Barnett, M. J., Fisher, R. F., Jones, T., Komp, C., Abola, A. P., Barloy-Hubler, F., Bowser, L., Capela, D., Galibert, F., Gouzy, J., Gurjal, M., Hong, A., Huizar, L., Hyman, R. W., Kahn, D., Kahn, M. L., Kalman, S., Keating, D. H., Palm, C., Peck, M. C., Surzycki, R., Wells, D. H., Yeh, K.-C., Davis, R. W., Federspiel, N. A., Long, S. R. (2001). Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid. Proc. Natl. Acad. Sci. USA 10.1073/pnas.161294798v1 [Abstract] [Full Text]  
• Sun, L. V., Foster, J. M., Tzertzinis, G., Ono, M., Bandi, C., Slatko, B. E., O'Neill, S. L. (2001). Determination of Wolbachia Genome Size by Pulsed-Field Gel Electrophoresis. J. Bacteriol. 183: 2219-2225 [Abstract] [Full Text]  
• Fischer, H.-M., Velasco, L., Delgado, M. J., Bedmar, E. J., Schären, S., Zingg, D., Göttfert, M., Hennecke, H. (2001). One of Two hemN Genes in Bradyrhizobium japonicum Is Functional during Anaerobic Growth and in Symbiosis. J. Bacteriol. 183: 1300-1311 [Abstract] [Full Text]  
• Göttfert, M., Röthlisberger, S., Kündig, C., Beck, C., Marty, R., Hennecke, H. (2001). Potential Symbiosis-Specific Genes Uncovered by Sequencing a 410-Kilobase DNA Region of the Bradyrhizobium japonicum Chromosome. J. Bacteriol. 183: 1405-1412 [Abstract] [Full Text]  
• Kurland, C. G., Andersson, S. G. E. (2000). Origin and Evolution of the Mitochondrial Proteome. Microbiol. Mol. Biol. Rev. 64: 786-820 [Abstract] [Full Text]  
• Martin-Didonet, C. C. G., Chubatsu, L. S., Souza, E. M., Kleina, M., Rego, F. G. M., Rigo, L. U., Yates, M. G., Pedrosa, F. O. (2000). Genome Structure of the Genus Azospirillum. J. Bacteriol. 182: 4113-4116 [Abstract] [Full Text]  
• Berg, O. G., Kurland, C. G. (2000). Why Mitochondrial Genes are Most Often Found in Nuclei. Mol Biol Evol 17: 951-961 [Abstract] [Full Text]  
• Nienaber, A., Huber, A., Göttfert, M., Hennecke, H., Fischer, H.-M. (2000). Three New NifA-Regulated Genes in the Bradyrhizobium japonicum Symbiotic Gene Region Discovered by Competitive DNA-RNA Hybridization. J. Bacteriol. 182: 1472-1480 [Abstract] [Full Text]  
• Bauer, E., Kaspar, T., Fischer, H.-M., Hennecke, H. (1998). Expression of the fixR-nifA Operon in Bradyrhizobium japonicum Depends on a New Response Regulator, RegR. J. Bacteriol. 180: 3853-3863 [Abstract] [Full Text]  
• Rodríguez, C., Romero, D. (1998). Multiple Recombination Events Maintain Sequence Identity Among Members of the Nitrogenase Multigene Family in Rhizobium etli. Genetics 149: 785-794 [Abstract] [Full Text]  
• Jumas-Bilak, E., Michaux-Charachon, S., Bourg, G., Ramuz, M., Allardet-Servent, A. (1998). Unconventional Genomic Organization in the Alpha Subgroup of the Proteobacteria. J. Bacteriol. 180: 2749-2755 [Abstract] [Full Text]  
• Barnett, M. J., Fisher, R. F., Jones, T., Komp, C., Abola, A. P., Barloy-Hubler, F., Bowser, L., Capela, D., Galibert, F., Gouzy, J., Gurjal, M., Hong, A., Huizar, L., Hyman, R. W., Kahn, D., Kahn, M. L., Kalman, S., Keating, D. H., Palm, C., Peck, M. C., Surzycki, R., Wells, D. H., Yeh, K.-C., Davis, R. W., Federspiel, N. A., Long, S. R. (2001). From the Cover: Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid. Proc. Natl. Acad. Sci. USA 98: 9883-9888 [Abstract] [Full Text]