This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Evans, D G Articles by Lee, C H Search for Related Content PubMed PubMed Citation Articles by Evans, D G Articles by Lee, C H

research-article

Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori.

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.

ABSTRACT

Gene hpaA, which codes for the receptor-binding subunit of the N-acetylneuraminyllactose-binding fibrillar hemagglutinin (NLBH) of Helicobacter pylori, was cloned and sequenced. The protein expressed by hpaA, designated HpaA, was identified as the adhesin subunit on the basis of its fetuin-binding activity and its reactivity with a polyclonal, monospecific rabbit serum prepared against NLBH purified from H. pylori. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and Western blots (immunoblots) showed that the cloned adhesin has the same molecular weight (20,000) as that found on H. pylori. Also, HpaA contains a short sequence of amino acids (KRTIQK) which are all either identical or functionally similar to those which compose the sialic acid-binding motif of Escherichia coli SfaS, K99, and CFA/I. Affinity-purified antibody specific for a 12-residue synthetic peptide that included this sequence blocked the hemagglutinating activity of H. pylori and was shown by immuno-gold electron microscopy to react with almost transparent material on unstained H. pylori cells, which is consistent with previous observations concerning the location and morphology of the NLBH.

This article has been cited by other articles:

• Osaki, T., Hanawa, T., Manzoku, T., Fukuda, M., Kawakami, H., Suzuki, H., Yamaguchi, H., Yan, X., Taguchi, H., Kurata, S., Kamiya, S. (2006). Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of a Mongolian gerbil model.. J Med Microbiol 55: 1477-1485 [Abstract] [Full Text]  
• Nurgalieva, Z. Z., Conner, M. E., Opekun, A. R., Zheng, C. Q., Elliott, S. N., Ernst, P. B., Osato, M., Estes, M. K., Graham, D. Y. (2005). B-Cell and T-Cell Immune Responses to Experimental Helicobacter pylori Infection in Humans. Infect. Immun. 73: 2999-3006 [Abstract] [Full Text]  
• Voland, P., Hafsi, N., Zeitner, M., Laforsch, S., Wagner, H., Prinz, C. (2003). Antigenic Properties of HpaA and Omp18, Two Outer Membrane Proteins of Helicobacter pylori. Infect. Immun. 71: 3837-3843 [Abstract] [Full Text]  
• Su, B., Ceponis, P. J. M., Lebel, S., Huynh, H., Sherman, P. M. (2003). Helicobacter pylori Activates Toll-Like Receptor 4 Expression in Gastrointestinal Epithelial Cells. Infect. Immun. 71: 3496-3502 [Abstract] [Full Text]  
• Eaton, K. A., Gilbert, J. V., Joyce, E. A., Wanken, A. E., Thevenot, T., Baker, P., Plaut, A., Wright, A. (2002). In Vivo Complementation of ureB Restores the Ability of Helicobacter pylori To Colonize. Infect. Immun. 70: 771-778 [Abstract] [Full Text]  
• Ryan, P. A., Pancholi, V., Fischetti, V. A. (2001). Group A Streptococci Bind to Mucin and Human Pharyngeal Cells through Sialic Acid-Containing Receptors. Infect. Immun. 69: 7402-7412 [Abstract] [Full Text]  
• McCarter, L. L. (2001). Polar Flagellar Motility of the Vibrionaceae. Microbiol. Mol. Biol. Rev. 65: 445-462 [Abstract] [Full Text]  
• Kumagai, T., Yan, J., Graham, D. Y., Tozuka, M., Okimura, Y., Ikeno, T., Sugiyama, A., Katsuyama, T., Ota, H. (2001). Serum Immunoglobulin G Immune Response to Helicobacter pylori Antigens in Mongolian Gerbils. J. Clin. Microbiol. 39: 1283-1288 [Abstract] [Full Text]  
• Thoreson, A.-C. E., Hamlet, A., Çelik, J., Byström, M., Nyström, S., Olbe, L., Svennerholm, A.-M. (2000). Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects. J. Clin. Microbiol. 38: 3436-3441 [Abstract] [Full Text]  
• Homuth, G., Domm, S., Kleiner, D., Schumann, W. (2000). Transcriptional Analysis of Major Heat Shock Genes of Helicobacter pylori. J. Bacteriol. 182: 4257-4263 [Abstract] [Full Text]  
• Karlsson, K.-A. (2000). The human gastric colonizer Helicobacter pylori: a challenge for host-parasite glycobiology. Glycobiology 10: 761-771 [Abstract] [Full Text]  
• Miller-Podraza, H., Bergstrom, J., Teneberg, S., Milh, M. A., Longard, M., Olsson, B.-M., Uggla, L., Karlsson, K.-A. (1999). Helicobacter pylori and Neutrophils: Sialic Acid-Dependent Binding to Various Isolated Glycoconjugates. Infect. Immun. 67: 6309-6313 [Abstract] [Full Text]  
• Marais, A., Mendz, G. L., Hazell, S. L., Megraud, F. (1999). Metabolism and Genetics of Helicobacter pylori: the Genome Era. Microbiol. Mol. Biol. Rev. 63: 642-674 [Abstract] [Full Text]  
• TSANG, K. W., LAM, S.-K., LAM, W.-K., KARLBERG, J., WONG, B. C., HU, W. H., YEW, W.-W., IP, M. S. (1998). High Seroprevalence of Helicobacter pylori in Active Bronchiectasis. Am. J. Respir. Crit. Care Med. 158: 1047-1051 [Abstract] [Full Text]  
• Huesca, M., Goodwin, A., Bhagwansingh, A., Hoffman, P., Lingwood, C. A. (1998). Characterization of an Acidic-pH-Inducible Stress Protein (hsp70), a Putative Sulfatide Binding Adhesin, from Helicobacter pylori. Infect. Immun. 66: 4061-4067 [Abstract] [Full Text]  
• Hayashi, S., Sugiyama, T., Amano, K.-I., Isogai, H., Isogai, E., Aihara, M., Kikuchi, M., Asaka, M., Yokota, K., Oguma, K., Fujii, N., Hirai, Y. (1998). Effect of Rebamipide, a Novel Antiulcer Agent, on Helicobacter pylori Adhesion to Gastric Epithelial Cells. Antimicrob. Agents Chemother. 42: 1895-1899 [Abstract] [Full Text]  
• Mattsson, A., Quiding-Jarbrink, M., Lonroth, H., Hamlet, A., Ahlstedt, I., Svennerholm, A.-M. (1998). Antibody-Secreting Cells in the Stomachs of Symptomatic and Asymptomatic Helicobacter pylori-Infected Subjects. Infect. Immun. 66: 2705-2712 [Abstract] [Full Text]  
• Mattsson, A., Tinnert, A., Hamlet, A., Lönroth, H., Bölin, I., Svennerholm, A.-M. (1998). . CVI 5: 288-293 [Abstract] [Full Text]  
• Namavar, F., Sparrius, M., Veerman, E. C. I., Appelmelk, B. J., Vandenbroucke-Grauls, C. M. J. E. (1998). Neutrophil-Activating Protein Mediates Adhesion of Helicobacter pylori to Sulfated Carbohydrates on High-Molecular-Weight Salivary Mucin. Infect. Immun. 66: 444-447 [Abstract] [Full Text]  
• Boren, T., Falk, P. (1994). Response. Science 264: 1387-1388  
• Boren, T, Falk, P, Roth, K., Larson, G, Normark, S (1993). Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens. Science 262: 1892-1895 [Abstract]