This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tan, H Articles by Chater, K F Search for Related Content PubMed PubMed Citation Articles by Tan, H Articles by Chater, K F

 Previous Article  |  Next Article 

J Bacteriol. 1993 February; 175(4): 933-940

research-article

Two developmentally controlled promoters of Streptomyces coelicolor A3(2) that resemble the major class of motility-related promoters in other bacteria.

John Innes Institute, John Innes Centre, Norwich, United Kingdom.

ABSTRACT

Experiments were designed to allow isolation of Streptomyces coelicolor promoters that depend on the whiG sporulation gene, which encodes a putative sigma factor important in the sporulation of aerial hyphae. The strategy, based on earlier evidence that sigma WhiG is limiting for sporulation (K. F. Chater, C. J. Burton, K. A. Plaskitt, M. J. Buttner, C. Méndez, and J. Helmann, Cell 59:133-143, 1989) was to seek DNA fragments that inhibit sporulation in aerial hyphae when present at a high copy number. In a suitable Sau3AI-generated library of DNA from S. coelicolor A3(2), two inserts were found to inhibit sporulation. Both inserts caused expression of the adjacent xylE reporter gene present in the vector in a developmentally normal strain of S. coelicolor, but there was no xylE expression in an otherwise isogenic whiG mutant. S1 nuclease protection experiments were done with RNAs isolated from these plasmid-bearing strains or from the wild-type strain lacking either recombinant plasmid. In each case, an apparent transcription start site was found upstream of an apparent open reading frame (ORF) and just downstream of sequences that resemble consensus features of promoters for motility-related genes in Bacillus subtilis and coliform bacteria. Such promoters depend on sigma factors (sigma D and sigma F, respectively) particularly similar to the deduced whiG gene product. Each of the putative whiG-dependent promoters is within an ORF that is upstream of, and potentially translationally coupled to, the putative whiG-dependent ORF (although use of one of the promoters would necessitate the use of a different start codon, further downstream). Thus, in unknown circumstances, the whiG-dependent ORFs may be expressed from a more remote promoter as part of a complex transcription unit.

This article has been cited by other articles:

• Tian, Y., Fowler, K., Findlay, K., Tan, H., Chater, K. F. (2007). An Unusual Response Regulator Influences Sporulation at Early and Late Stages in Streptomyces coelicolor. J. Bacteriol. 189: 2873-2885 [Abstract] [Full Text]  
• Roth, V., Aigle, B., Bunet, R., Wenner, T., Fourrier, C., Decaris, B., Leblond, P. (2004). Differential and Cross-Transcriptional Control of Duplicated Genes Encoding Alternative Sigma Factors in Streptomyces ambofaciens. J. Bacteriol. 186: 5355-5365 [Abstract] [Full Text]  
• Kwak, J., Dharmatilake, A. J., Jiang, H., Kendrick, K. E. (2001). Differential Regulation of ftsZ Transcription during Septation of Streptomyces griseus. J. Bacteriol. 183: 5092-5101 [Abstract] [Full Text]  
• Jiang, H., Kendrick, K. E. (2000). Characterization of ssfR and ssgA, Two Genes Involved in Sporulation of Streptomyces griseus. J. Bacteriol. 182: 5521-5529 [Abstract] [Full Text]  
• Kelemen, G. H., Brian, P., Flärdh, K., Chamberlin, L., Chater, K. F., Buttner, M. J. (1998). Developmental Regulation of Transcription of whiE, a Locus Specifying the Polyketide Spore Pigment in Streptomyces coelicolor A3(2). J. Bacteriol. 180: 2515-2521 [Abstract] [Full Text]