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Participation of RAP1 protein in expression of the Saccharomyces cerevisiae arginase (CAR1) gene.

Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.

ABSTRACT

Regulated expression of the inducible arginase (CAR1) gene of Saccharomyces cerevisiae has been shown to require three upstream activation sequences (UASs) and an upstream repression sequence, URS1. Two of the UAS elements, UASC1 and UASC2, operate in an inducer-independent manner, while the third, UASI, is inducer dependent. UASC1 and UASC2 were previously shown to contain ABF-1 binding sites that were required for normal transcription. In this work, we demonstrate that UASC1 and UASC2 also contain two and three sites, respectively, that are able to bind RAP1 protein. RAP1 binding to these sites, however, is significantly weaker than that to sites in TEF2 and HMRE. The effects of mutating the sites individually or in combination suggest that at least three of them, two in UASC1 and one in UASC2, probably participate in CAR1 expression.

This article has been cited by other articles:

• Park, H.-D., Scott, S., Rai, R., Dorrington, R., Cooper, T. G. (1999). Synergistic Operation of the CAR2 (Ornithine Transaminase) Promoter Elements in Saccharomyces cerevisiae. J. Bacteriol. 181: 7052-7064 [Abstract] [Full Text]